Enhanced Insecticidal Activity of Chlorfenapyr against Spodoptera frugiperda by Reshaping the Intestinal Microbial Community and Interfering with the Metabolism of Iron-Based Metal–Organic Frameworks

• Published in: ACS applied materials & interfaces Vol. 15; no. 30; pp. 36036 - 36051
• Main Authors: Chen, Huiya, Yang, Liupeng, Zhou, Jingtong, Liu, Pengpeng, Zhu, Shiqi, Li, Youzhi, Huang, Suqing, Xu, HanHong, Zhang, Zhixiang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.08.2023
• Subjects: Animals; Biological and Medical Applications of Materials and Interfaces; Insecticides - pharmacology; Iron - pharmacology; Larva; Metal-Organic Frameworks - pharmacology; Microbiota; RNA, Ribosomal, 16S; Spodoptera - genetics; Zea mays - genetics; Spodoptera frugiperda; microbiomics; metabolomics; metal−organic frameworks; surface functionalization
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.3c07598

Spodoptera frugiperda (S. frugiperda) is an invasive pest that threatens global crop production and food security and poses a serious threat to maize production worldwide. Metal–organic framework (MOF) nanocarriers have great potential for agricultural pest control applications. The present study successfully prepared the chemical cross-linking of iron-based metal–organic framework nanoparticles (MIL-101­(Fe)-NH2 NPs) with sodium lignosulfonate (SL) as a pH/laccase double stimuli-responsive pesticide release system. The average particle size of the prepared chlorfenapyr (CF)-loaded nanoparticles (CF@MIL-101-SL NPs) was 161.54 nm, and the loading efficiency was 44.52%. Bioactivity assays showed that CF@MIL-101-SL NPs increased the toxicity of CF to S. frugiperda and caused the rupture of the peritrophic membrane and enlargement of the midgut. Data from 16S rRNA gene sequencing showed that CF@MIL-101-SL treatment reduced the resistance of S. frugiperda to pesticides and pathogens and affected nutrient and energy availability by remodeling the intestinal microbiota of S. frugiperda. The dysregulated microbial community interacted with the broken peritrophic membrane, which exacerbated damage to the host. Nontargeted metabolomic results showed that ABC transporters may be a potential mechanism for the enhanced toxicity of CF@MIL-101-SL to S. frugiperda. In summary, the present study provides effective strategies for toxicological studies of nanopesticides against insects.