Structural and thermodynamic characterization of temperature-dependent changes in the folding pattern of a synthetic triamide

• Published in: Journal of the American Chemical Society Vol. 115; no. 10; pp. 4228 - 4245
• Main Authors: Dado, Gregory P, Gellman, Samuel H
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.05.1993; Amer Chemical Soc
• Subjects: Atomic and molecular physics; Barrier heights (internal rotation, inversion, rotational isomerism, conformational dynamics); Chemistry; Chemistry, Multidisciplinary; Exact sciences and technology; Molecular properties and interactions with photons; Physical Sciences; Physics; Properties of molecules and molecular ions; Science & Technology; SIMPLE OLIGOAMIDES; NUCLEAR MAGNETIC RESONANCE; HYDROGEN-BOND; ALANINE PEPTIDE; POTENTIALS; CARBON-TETRACHLORIDE; PROTEINS; CHLOROFORM; CYCLIC-PEPTIDES; CONFORMATIONS; Chemical solution; Temperature; Conformational dynamics; Overhauser effect; Experimental study; Chemical shift; Concentration effect; FTIR spectrum; Carboxamide; Infrared spectrum; Vibrational mode; NMR spectrum; Intramolecular hydrogen bond; Organic compounds
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja00063a046

Variable-temperature H-1 NMR and IR studies of triamide 1 and related compounds indicate that 1 undergoes dramatic temperature-dependent conformational changes in relatively nonpolar solvents (methylene chloride and chloroform). The folding pattern favored at low temperatures in these chlorocarbons (1c) contains a single C=O...H-N hydrogen bond in a nine-membered ring, while a folding pattern containing only a six-membered-ring C=O...N-H interaction (1a) is favored at higher temperatures. Dimethyl sulfoxide, a very strong hydrogen-bond-accepting solvent, disrupts all internal hydrogen bonding in 1. Acetonitrile appears to disrupt the six-membered-ring hydrogen bond selectively and to promote nine-membered-ring interaction at room temperature, relative to chlorocarbon solvents. By treating the behavior of 1 as a two-state system, in which folding pattern 1c is considered to be the ''native state'' and all other folding patterns comprise the ''denatured state'', we have been able to carry out van't Hoff analyses of the temperature-dependent conformational changes. In methylene chloride, the native state is enthalpically preferred by 1.9-2.5 kcal/mol but entropically disfavored by 7.4-9.1 eu. Similar values are obtained in chloroform. This thermodynamic characterization demonstrates that, even in a relatively nonpolar environment, the relative enthalpic stabilities of alternative folding patterns cannot be predicted simply by maximizing the pairing of hydrogen-bond donors and acceptors.