Cascade/Parallel Biocatalysis via Multi-enzyme Encapsulation on Metal–Organic Materials for Rapid and Sustainable Biomass Degradation

• Published in: ACS applied materials & interfaces Vol. 13; no. 36; pp. 43085 - 43093
• Main Authors: Li, Qiaobin, Pan, Yanxiong, Li, Hui, Lenertz, Mary, Reed, Kailyn, Jordahl, Drew, Bjerke, Taylor, Ugrinov, Angel, Chen, Bingcan, Yang, Zhongyu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.09.2021
• Subjects: Amylases - chemistry; Biocatalysis; Biomass; Enzymes, Immobilized - chemistry; Functional Inorganic Materials and Devices; Glucan 1,4-alpha-Glucosidase - chemistry; Hydrolysis; Metal-Organic Frameworks - chemistry; Peptide Hydrolases - chemistry; Proof of Concept Study; Proteins - chemistry; Starch - chemistry; metal−organic materials; protease protection; cascade biocatalysis; total dietary fiber quantification; metal−organic frameworks; enzyme immobilization; one-pot synthesis
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.1c12209

Multiple-enzyme cooperation simultaneously is an effective approach to biomass conversion and biodegradation. The challenge, however, lies in the interference of the involved enzymes with each other, especially when a protease is needed, and thus, the difficulty in reusing the enzymes; while extracting/synthesizing new enzymes costs energy and negative impact on the environment. Here, we present a unique approach to immobilize multiple enzymes, including a protease, on a metal–organic material (MOM) via co-precipitation in order to enhance the reusability and sustainability. We prove our strategy on the degradation of starch-containing polysaccharides (require two enzymes to degrade) and food proteins (require a protease to digest) before the quantification of total dietary fiber. As compared to the widely adopted “official” method, which requires the sequential addition of three enzymes under different conditions (pH/temperature), the three enzymes can be simultaneously immobilized on the surface of our MOM crystals to allow for contact with the large substrates (starch), while MOMs offer sufficient protection to the enzymes so that the reusability and long-term storage are improved. Furthermore, the same biodegradation can be carried out without adjusting the reaction condition, further reducing the reaction time. Remarkably, the simultaneous presence of all enzymes enhances the reaction efficiency by a factor of ∼3 as compared to the official method. To our best knowledge, this is the first experimental demonstration of using aqueous-phase co-precipitation to immobilize multiple enzymes for large-substrate biocatalysis. The significantly enhanced efficiency can potentially impact the food industry by reducing the labor requirement and enhancing enzyme cost efficiency, leading to reduced food cost. The reduced energy cost of extracting enzymes and adjusting reaction conditions minimize the negative impact on the environment. The strategy to prevent protease damage in a multi-enzyme system can be adapted to other biocatalytic reactions involving proteases.