Lysine-Sarcosine PiPo Functionalized Surface with Excellent Hemocompatibility

• Published in: ACS applied materials & interfaces Vol. 15; no. 25; pp. 29700 - 29712
• Main Authors: Hao, Kai, Cui, Ronglu, Fang, Lifeng, Ling, Jun, Zhu, Baoku
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 28.06.2023
• Subjects: Biological and Medical Applications of Materials and Interfaces; Lysine; Polyethylene Glycols - chemistry; Proteins - chemistry; Sarcosine; Surface Properties; Water - chemistry; polysarcosine; hemocompatibility; protein resistance; coating; mussel-inspired chemistry
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.2c19677

Polysarcosine (PSar) is an electrically neutral and excellently hydrophilic polypeptoid showing limited interaction with proteins and cells, which possesses better biocompatibility compared with polyethylene glycol. However, the immobilization of PSar is difficult due to the high water solubility. Herein, lysine-sarcosine PiPo, which was the random copolymer of lysine and sarcosine (PLS), was synthesized via a phosgene-free and water-tolerable polymerization of N-phenyloxycarbonyl-amino acids for the first time. PLS was immobilized by tannic acid (TA) on the polysulfone (PSf) membrane for a short time to obtain a neutral surface. The modified membrane showed improved hydrophilicity, decreased protein adsorption, and low cytotoxicity. Moreover, barely any hemolysis, no platelet adhesion, prolonged clotting time, and low complement activation further suggested good hemocompatibility. In order to improve the antifouling ability of the membrane under pressure, the neutral surface was oxidized by sodium periodate, which accelerated the chemical reaction between amino groups in PLS and phenolic hydroxyl groups in TA. Meanwhile, carboxyl groups generated due to the decomposition of TA and a negatively charged surface were obtained. While maintaining the good properties of the unoxidized one, the hydrophilicity of the oxidized membrane was improved and the clotting time was further prolonged. Besides, the filtration recovery of the oxidized membrane was improved remarkably. This approach of rapid immobilization of PSar has great potential for applications in the biomedical area, especially for blood-contacting materials.