Universal Fluorescence Biosensor Platform Based on Graphene Quantum Dots and Pyrene-Functionalized Molecular Beacons for Detection of MicroRNAs

• Published in: ACS applied materials & interfaces Vol. 7; no. 30; pp. 16152 - 16156
• Main Authors: Zhang, Huan, Wang, Yunsheng, Zhao, Daiwei, Zeng, Dongdong, Xia, Jiaoyun, Aldalbahi, Ali, Wang, Chenguang, San, Lili, Fan, Chunhai, Zuo, Xiaolei, Mi, Xianqiang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.08.2015
• Subjects: Biosensing Techniques - instrumentation; Equipment Design; Equipment Failure Analysis; Fluorescence Resonance Energy Transfer - instrumentation; Graphite - chemistry; MicroRNAs - analysis; MicroRNAs - genetics; Molecular Probe Techniques - instrumentation; Molecular Probes - chemistry; Pyrenes - chemistry; Quantum Dots; Reproducibility of Results; Sensitivity and Specificity; fluorescence resonance energy transfer; microRNA; biosensor; graphene quantum dots; pyrene
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.5b04773

A novel biosensor platform was developed for detection of microRNAs (miRNAs) based on graphene quantum dots (GQDs) and pyrene-functionalized molecular beacon probes (py-MBs). Pyrene was introduced to trigger specifically fluorescence resonance energy transfer (FRET) between GQDs and fluorescent dyes labeled on py-MBs, and the unique fluorescent intensity change produced a novel signal for detection of the target. The platform realized detection of miRNAs in a wide range from 0.1 nM to 200 nM with great discrimination abilities, as well as multidetection of different kinds of miRNAs, which paved a brand new way for miRNA detection based on GQDs.