Visible-Light Neural Stimulation on Graphitic-Carbon Nitride/Graphene Photocatalytic Fibers

• Published in: ACS applied materials & interfaces Vol. 9; no. 40; pp. 34736 - 34743
• Main Authors: Zhang, Zhongyang, Xu, Ruodan, Wang, Zegao, Dong, Mingdong, Cui, Bianxiao, Chen, Menglin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 11.10.2017
• Subjects: Catalysis; Graphite - chemistry; Light; Nitriles; electrospun fibers; visible-light photocatalytic; graphitic carbon nitride; graphene; neural stimulator
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.7b12733

Light stimulation allows remote and spatiotemporally accurate operation that has been applied as effective, noninvasive means of therapeutic interventions. Here, visible-light neural stimulation of graphitic carbon nitride (g-C3N4), an emerging photocatalyst with visible-light optoelectronic conversion, was for the first time investigated. Specifically, g-C3N4 was combined with graphene oxide (GO) in a three-dimensional manner on the surfaces of electrospun polycaprolactone/gelatin (PG) fibers and functioned as a biocompatible interface for visible-light stimulating neuronal differentiation. The enhanced photocatalytic function of g-C3N4 was realized by spreading g-C3N4 on GO coated electrospun (PG) microfibers to improve both charge separation and surface area. Ascorbic acid (AA) was used in the cell culture medium not only as a photoexcited hole scavenger but also as a mediator of GO reduction to further improve the electrical conductivity. The successful coatings of g-C3N4, GO, and AA-mediated GO reduction were confirmed using scanning electron microscopy, photoluminescence, Raman spectroscopy, and X-ray photoelectron spectroscopy. Biocompatibility of g-C3N4 (0.01–0.9 mg/mL) to PC12 cells was confirmed by the lactate dehydrogenase (LDH) assay, Live–Dead staining, and colorimetric cell viability assay CCK-8. Under a bidaily, monochromatic light stimulation at a wavelength of 450 nm at 10 mW/cm2, a 18.5-fold increase of neurite outgrowth of PC12 was found on g-C3N4-coated fibers, while AA-reduced GO–g-C3N4 hybrid brought a further 2.6-fold increase, suggesting its great potential as a visible-light neural stimulator that could optically enhance neural growth in a spatiotemporal-specific manner.