Amino Acids Separations by Displacement Chromatography. Effects of Homogeneous and Heterogeneous Equilibria on Separation Performances

• Published in: Industrial & engineering chemistry research Vol. 39; no. 7; pp. 2468 - 2479
• Main Authors: Zammouri, Amel, Muhr, Laurence, Grevillot, Georges
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 03.07.2000
• Subjects: Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Others; Proteins; Various methods and equipments; Phenylalanine; Preparative chromatography; Anionic resin; Carbon dioxide; Theoretical study; Selectivity; Glycine; Dissolution; Modeling; Ion exchange chromatography; Binary mixture; Separation method; Chemical shift; Histidine; Lysine; Aminoacid; Mathematical model; Performance
• ISSN: 0888-5885; 1520-5045
• DOI: 10.1021/ie990810n

In this paper we investigate displacement chromatography of amino acids on columns of strong anionic resins in the hydroxyl form. The displacer used here is carbon dioxide dissolved in water, but the theory is also valid to other types of displacers. A theoretical study of the effect of both dissociation equilibria in solution and ion-exchange equilibria is presented. It is shown that the separation quality depends not only on the selectivity coefficient but also on a term relative to homogeneous equilibria in the liquid phase. This factor is related to the ratio of the second amino acid deprotonation constants.