Assessing Potential Bioavailability of Raspberry Anthocyanins Using an in Vitro Digestion System

• Published in: Journal of agricultural and food chemistry Vol. 53; no. 15; pp. 5896 - 5904
• Main Authors: McDougall, Gordon J, Dobson, Patricia, Smith, Pauline, Blake, Alison, Stewart, Derek
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 27.07.2005
• Subjects: anthocyanins; Anthocyanins - analysis; Anthocyanins - pharmacokinetics; bioavailability; Biological and medical sciences; Biological Availability; Digestion; extracts; Feeding. Feeding behavior; Food industries; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; in vitro digestion; In Vitro Techniques; raspberries; Rosaceae - chemistry; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Anthocyanin; polyphenols; Stability; Fruit; Health; antioxidants; Berry; Bioavailability; Digestion; Antioxidant; Flavonoid; In vitro; Polyphenol; Quality; Raspberry; Pigments; Anthocyanins; health benefits
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf050131p

The bioavailability of anthocyanins from raspberry extracts was assessed using an in vitro digestion procedure that mimics the physiochemical and biochemical changes that occur in the upper gastrointestinal tract (GIT). Effectively all of the total phenol content of the raspberry extract survived gastric digestion and partitioned between the IN sample, which represents the serum available material, and the OUT sample, which represents the material that remains in the GIT and passes through to the colon. All of the anthocyanins also survived gastric digestion, but only ∼5% entered the IN sample and ∼70% of total anthocyanins were recovered in the IN and OUT samples. Codigestion of the raspberry extract with commonly combined foodstuffs such as bread, breakfast cereal, ice cream, and cooked minced beef gave a different pattern. The total phenol content of the IN samples was slightly reduced by codigestion with ice cream or breakfast cereal but unaffected by codigestion with bread or minced beef. In most cases, the phenol contents of the postgastric and OUT samples were reduced as compared with the expected values. However, the anthocyanin content of the IN samples was unaffected or increased by coincubation with the foodstuffs. This suggests that polyphenols transiently bind to food matrices during digestion, which protects the more labile anthocyanins from degradation, and they are free to diffuse into the IN sample. The anthocyanin composition of the bioavailability samples was monitored by liquid chromatography−mass spectrometry. All eight anthocyanins previously identified in raspberry were detected in the extract and the postgastric samples at similar yields. All eight anthocyanins could be discerned in the IN and OUT samples, but some such as cyanidin-3-O-glucoside were greatly reduced and others such as pelargonidin-3-O-glucoside were apparently increased in abundance. These differences in stability and their importance for the bioavailability of anthocyanins are discussed. Keywords: Anthocyanins; antioxidants; berry; bioavailability; health benefits; raspberry; polyphenols; stability