Unraveling the Molecular Mechanisms of Trehalose-Mediated Protection and Stabilization of Escherichia coli Lipid Membrane during Desiccation

• Published in: The journal of physical chemistry. B Vol. 127; no. 20; pp. 4496 - 4507
• Main Authors: Maiti, Archita, Daschakraborty, Snehasis
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 25.05.2023
• Subjects: B: Biophysical and Biochemical Systems and Processes; cryopreservation; Desiccation; Disaccharides; Escherichia coli; gels; hydrogen; lipid bilayers; Lipid Bilayers - chemistry; lipids; molecular dynamics; probiotics; trehalose; Trehalose - chemistry; vitrification; Water - chemistry
• ISSN: 1520-6106; 1520-5207; 1520-5207
• DOI: 10.1021/acs.jpcb.3c01730

Trehalose, a disaccharide renowned for its ability to stabilize biomolecular architectures under strenuous conditions, finds extensive use in the cryopreservation of probiotics. A profound comprehension of its molecular-level interactions is of great significance. It is notable that current research in the realm of lipid–sugar interactions primarily employs single-component lipid bilayers, which are far from being representative of real cell membranes. Our investigation, however, utilizes molecular dynamics simulations to delve into the specifics of a realistic Escherichia coli membrane that encompasses a diverse array of lipid types, comprising fourteen distinct species, subject to varying hydration levels. The results of our study showcase that the reduction of hydration levels induces lipid ordering and the formation of gel phases, yet trehalose, by forming hydrogen bonds with lipid headgroups, serves to uphold fluidity and supplant the role of water. Moreover, our findings evince that augmented trehalose concentrations lead to a slowdown in lipid motion and contribute to the maintenance of fluidity by way of endowing a viscous matrix. It is noteworthy that our conclusions lend support to the notion that water replacement and vitrification, despite their seemingly disparate nature, need not be considered mutually exclusive in a real bacterial membrane.