Effects of Selenite on Unicellular Green Microalga Chlorella pyrenoidosa: Bioaccumulation of Selenium, Enhancement of Photosynthetic Pigments, and Amino Acid Production

• Published in: Journal of agricultural and food chemistry Vol. 65; no. 50; pp. 10875 - 10883
• Main Authors: Zhong, Yu, Cheng, Jay J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.12.2017
• Subjects: Amino Acids - biosynthesis; Biomass; Catalase - metabolism; Chlorella - growth & development; Chlorella - metabolism; Glutathione Peroxidase - metabolism; Microalgae - growth & development; Microalgae - metabolism; Photosynthesis; Pigments, Biological - biosynthesis; Reactive Oxygen Species - metabolism; Selenious Acid - metabolism; Selenium - metabolism; Chlorella pyrenoidosa; bioaccumulation; selenium; antioxidants; fatty acid profile; amino acids
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b04246

Microalgae were studied as function bioaccumulators of selenium (Se) for food and feed supplement. To investigate the bioaccumulation of Se and its effects on the unicellular green alga Chlorella pyrenoidosa, the algal growth curve, fluorescence parameters, antioxidant enzyme activity, and fatty acid and amino acid profiles were examined. We found that Se at low concentrations (≤40 mg L–1) positively promoted algal growth and inhibited lipid peroxidation and intracellular reactive oxygen species. The antioxidative effect was associated with an increase in the levels of glutathione peroxidase, catalase, linolenic acid, and photosynthetic pigments. Meanwhile, a significant increase in amino acid and organic Se content was also detected in the microalgae. In contrast, we found opposite effects in C. pyrenoidosa exposed to >60 mg L–1 Se. The antioxidation and toxicity appeared to be correlated with the bioaccumulation of excess Se. These results provide a better understanding of the effect of Se on green microalgae, which may help in the development of new technological applications for the production of Se-enriched biomass from microalgae.