Investigation of Two Species of Huang-qi (Aatragalusmembranaceus and Hedysarum polybotrys) by HPLC, ITS,Microscopic Morphology and Antioxidant Activities

Hedysarum polybotrys is commonly substituted for Astragalus membranaceus in Taiwan for many years. This study described several different ways, including microscopic morphology, ITS (internal transcribed spacer) sequencing and HPLC fingerprint methods to identify these two species. Furthermore, the...

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Published inYàowu shi͡p︡in fenxi Vol. 20; no. 3; pp. 603 - 610
Main Authors LEE, I-JUNG, HUANG, PIN-CHIEH, ZHANG, LI-JIE, LIAW, CHIA-CHING, LIN, MING-REN
Format Journal Article
LanguageEnglish
Chinese
Published 台灣 衛生福利部食品藥物管理署 01.09.2012
Food and Drug Administration
Subjects
antioxidant activity
Astragali Radix
Astragalus membranaceus
Hedysarum polybotrys
HPLC fingerprint
ITS
MEDLINE
microscopic
SCIE
Scopus
Astragali Radix
HPLC fingerprint
ITS
microscopic
antioxidant activity
Astragalus membranaceus
Hedysarum polybotrys
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Summary:Hedysarum polybotrys is commonly substituted for Astragalus membranaceus in Taiwan for many years. This study described several different ways, including microscopic morphology, ITS (internal transcribed spacer) sequencing and HPLC fingerprint methods to identify these two species. Furthermore, the antioxidant activities of the two plants were evaluated and H. polybotrys had higher antioxidant activity than A. membranaceus. All the methods gave consistent conclusions that test samples KRA2, 3 and 8 belonged to A. membranaceus, while KRA1, 4, 5, 6 and 7 belonged to H. polybotrys. The results revealed that all the above methods may successfully discriminate between A. membranaceus and H. polybotrys.
ISSN:1021-9498
2224-6614
DOI:10.6227/jfda.2012200306