Temperature-Dependent Bioaccumulation, Metabolism, and Hepatotoxicity of Flufiprole in Lizards (Eremias argus)

• Published in: Environmental science & technology Vol. 57; no. 31; pp. 11612 - 11625
• Main Authors: Chang, Jing, An, Qiong, Xie, Yun, Liu, Wentao, Xu, Peng, Hao, Weiyu, Wan, Bin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 08.08.2023
• Subjects: Agricultural land; Animals; Bioaccumulation; Chemical and Drug Induced Liver Injury; Climate change; Deoxyguanosine; Eremias argus; Exposure; Fipronil; Global Warming; Glutathione; Hepatotoxicity; Humans; Insecticides; Insecticides - metabolism; Insecticides - toxicity; Lesions; Liver; Lizards; Lizards - metabolism; Metabolism; Metabolites; Microsomes; Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants; Oxidation; Pesticides; Phenylpyrazole; Pyrazoles - metabolism; Pyrazoles - toxicity; Risk Assessment; Soil contamination; Soil Pollutants - metabolism; Soil Pollutants - toxicity; Soil pollution; Soil temperature; Squamata; Temperature; Temperature dependence; oxidative damage; metabolism; global warming; phenylpyrazole insecticide; reptiles
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/acs.est.3c01049

As a phenylpyrazole insecticide, flufiprole is an important substitute for fipronil in the agricultural field of China. However, its bioaccumulation and metabolism in terrestrial organisms especially in the lizards living in the agricultural area have rarely been investigated. As an ectothermic animal, lizards are also sensitive to temperature changes. Considering global warming, this study measured bioaccumulation, metabolism, and hepatotoxicity of flufiprole in the Chinese native lizard (Eremias argus) under different temperature stresses. Lizards exposed to flufiprole-contaminated soil adsorbed flufiprole through the skin and flufiprole was preferred to accumulate in lizard liver and brain. The oxidation product fipronil sulfone was the main metabolite of flufiprole in both lizard liver and human liver microsomes, which were mainly metabolized by lizard CYP3A19 or human CYP3A4. The fipronil sulfone concentration increased with increased temperature in lizard tissues. In addition, more serious oxidative damage was shown under higher temperature as the glutathione (GSH), malondialdehyde (MDA), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels in lizards increased with increased temperature after flufiprole exposure. Flufiprole exposure also induced lizard liver lesions, and these lesions became more serious in the higher-temperature groups. This study provided new insights into the risk assessment of flufiprole in lizards under global warming.