A New Nanobiocatalytic System Based on Allosteric Effect with Dramatically Enhanced Enzymatic Performance

• Published in: Journal of the American Chemical Society Vol. 135; no. 4; pp. 1272 - 1275
• Main Authors: Wang, Liang-Bing, Wang, You-Cheng, He, Rong, Zhuang, Awei, Wang, Xiaoping, Zeng, Jie, Hou, J. G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 30.01.2013
• Subjects: Allosteric Regulation; Amylases - chemistry; Amylases - metabolism; Biocatalysis; Calcium Phosphates - chemistry; Calcium Phosphates - metabolism; Nanotechnology; Organometallic Compounds - chemistry; Organometallic Compounds - metabolism; Particle Size; Surface Properties
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja3120136

We report a rational design of CaHPO4-α-amylase hybrid nanobiocatalytic system based on allosteric effect and an explanation of the increase in catalytic activity when certain enzymes are immobilized in specific nanomaterials. Employing a calcification approach in aqueous solutions, we acquired such new nanobiocatalytic systems with three different morphologies, i.e., nanoflowers, nanoplates, and parallel hexahedrons. Through studying enzymatic performance of these systems and free α-amylase with/without Ca2+, we demonstrated how two factors, allosteric regulation and morphology of the as-synthesized nanostructures, predominantly influence enzymatic activity. Benefiting from both the allosteric modulation and its hierarchical structure, CaHPO4-α-amylase hybrid nanoflowers exhibited dramatically enhanced enzymatic activity. As a bonus, the new system we devised was found to enjoy higher stability and durability than free α-amylase plus Ca2+.