Molecular Reprogramming of Arabidopsis in Response to Perturbation of Jasmonate Signaling

• Published in: Journal of proteome research Vol. 13; no. 12; pp. 5751 - 5766
• Main Authors: Yan, Huizhuan, Yoo, Mi-Jeong, Koh, Jin, Liu, Lihong, Chen, Yazhou, Acikgoz, Dogukan, Wang, Qiaomei, Chen, Sixue
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.12.2014
• Subjects: Acetates - pharmacology; Amino Acid Sequence; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Chromatography, Liquid; Cyclopentanes - metabolism; Cyclopentanes - pharmacology; Gas Chromatography-Mass Spectrometry; Gene Expression Profiling - methods; Gene Expression Regulation, Plant - drug effects; Glucose - metabolism; Mass Spectrometry; Metabolome - drug effects; Metabolomics - methods; Molecular Sequence Data; Mutation; Oxylipins - metabolism; Oxylipins - pharmacology; Plant Growth Regulators - pharmacology; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - metabolism; Plant Shoots - drug effects; Plant Shoots - genetics; Plant Shoots - metabolism; Repressor Proteins - genetics; Repressor Proteins - metabolism; Sequence Homology, Amino Acid; Signal Transduction - drug effects; Transcriptome - drug effects; Arabidopsis thaliana; transcriptomics; JAZ; metabolomics; jasmonate; molecular networks
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/pr500739v

Jasmonates (JAs) are important phytohormones that regulate a wide range of plant processes including growth, development, senescence, and defense. Jasmonate ZIM-domain (JAZ) proteins are repressors in JA signaling. In Arabidopsis thaliana, 12 JAZ encoding genes were identified, but only a few have been studied in detail. In this study, we focused on characterizing the molecular networks involving JAZ2 and JAZ7. To understand the phenotypes and elucidate the regulatory functions of JAZ2 and JAZ7, shoot and root tissues from wild type (WT), jaz2, and jaz7 were harvested for RNA sequencing and metabolomics. Distinct changes of transcripts and metabolites in JA biosynthesis, primary and specialized metabolism, and oxidative stress were observed among the three genotypes. In particular, many defense or stress-associated metabolites and specialized metabolites were increased in response to methyl jasmonate (MeJA) treatment. Most importantly, these changes were subjected to quantitative modulation by the JAZ proteins at both transcriptional and metabolic levels, the degree of which may control resource allocation between growth and defense. This study not only reveals MeJA-induced molecular reprogramming but also demonstrates the functions of JAZ proteins as key regulators in fine-tuning JA signal transduction.