Geometric Control of Cell Alignment and Spreading within the Confinement of Antiadhesive Poly(Ethylene Glycol) Microstructures on Laser-Patterned Surfaces

In this study, a mask-less laser-assisted patterning method is used to fabricate well-defined cell-adhesive microdomains delimited by protein-repellent poly­(ethylene glycol) (PEG) microstructures prepared from multiarm (8-PEG) macromonomers. The response of murine fibroblasts (L-929) toward these m...

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Published inACS biomaterials science & engineering Vol. 1; no. 9; pp. 747 - 752
Main Authors Strehmel, Christine, Perez-Hernandez, Heidi, Zhang, Zhenfang, Löbus, Axel, Lasagni, Andrés F, Lensen, Marga C
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 14.09.2015
Subjects
geometry
microstructure
poly(ethylene glycol)
cell adhesion
laser-assisted patterning
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Summary:In this study, a mask-less laser-assisted patterning method is used to fabricate well-defined cell-adhesive microdomains delimited by protein-repellent poly­(ethylene glycol) (PEG) microstructures prepared from multiarm (8-PEG) macromonomers. The response of murine fibroblasts (L-929) toward these microdomains is investigated, revealing effective cell confinement within the cell-adhesive areas surrounded by nonadhesive 8-PEG microstructures. Moreover, the spatial positioning of cells in microdomains of various sizes and geometries is analyzed, indicating control of cell density, size, and elongated cell shape induced by the size of the microdomains and the geometric confinement.
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ISSN:2373-9878
2373-9878
DOI:10.1021/ab5001657