Proteome Analysis of Cold Acclimation in Sunflower

Cold acclimation is the phenomenon in which plants are exposed to low, but nonfreezing, temperatures before exposure to drastic temperatures. To investigate how sunflower plants adjust their metabolism during cold treatment, a comparative proteomic approach, based on spectral counting data, was adop...

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Published inJournal of proteome research Vol. 10; no. 5; pp. 2330 - 2346
Main Authors Balbuena, Tiago S, Salas, Joaquín J, Martínez-Force, Enrique, Garcés, Rafael, Thelen, Jay J
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 06.05.2011
Subjects
Acclimatization - genetics
Chromatography, Liquid
Cluster Analysis
Cold Temperature
Computational Biology
Electrophoresis, Polyacrylamide Gel
Gene Expression Profiling
Gene Expression Regulation, Plant - genetics
Helianthus - genetics
Helianthus - metabolism
Plant Proteins - metabolism
Proteomics - methods
Tandem Mass Spectrometry
mass spectrometry
GeLC−MS
cold acclimation
spectral counting
Helianthus annuus
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Summary:Cold acclimation is the phenomenon in which plants are exposed to low, but nonfreezing, temperatures before exposure to drastic temperatures. To investigate how sunflower plants adjust their metabolism during cold treatment, a comparative proteomic approach, based on spectral counting data, was adopted to identify differentially expressed proteins in leaves of freezing susceptible (Hopi) and tolerant (PI 543006 and BSD-2-691) lines after cold acclimation. In total 718, 675, and 769 proteins were confidently identified by tandem mass spectrometry in Hopi, PI 543006, and BSD-2-691 sunflower lines. Tolerant lines PI 543006 and BSD-2-691 showed the highest number of differentially expressed proteins, as 43, 72, and 168 proteins changed their expression in Hopi, PI 543006, and BSD-2-691 sunflower lines, respectively, at 95% confidence. Cold-responsive proteins were mostly involved in metabolism, protein synthesis, energy, and defense processes in all sunflower lines studied. Hierarchical clustering of all differentially expressed proteins resulted in the characterization of 14 different patterns of expression across Hopi, PI 543006, and BSD-2-691 and indicated that tolerant lines showed different proteome responses to cold acclimation.
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ISSN:1535-3893
1535-3907
DOI:10.1021/pr101137q