Modeling the Electrostatic Signature of Single Enzyme Activity

Charge sensors based on nanoscale field-effect transistors are a promising new tool to probe the dynamics of individual enzymes. However, it is currently unknown whether the electrostatic signals associated with biological activity exceed detection limits. We report calculations of electrostatic sig...

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Bibliographic Details
Published inThe journal of physical chemistry. B Vol. 114; no. 9; pp. 3330 - 3333
Main Authors Prisbrey, Landon, Schneider, Guenter, Minot, Ethan
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 11.03.2010
Subjects
B: Biophysical Chemistry
Computer Simulation
Deoxyribonuclease I - chemistry
Electrolytes - chemistry
Models, Molecular
Muramidase - chemistry
Nanotubes, Carbon - chemistry
Static Electricity
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Summary:Charge sensors based on nanoscale field-effect transistors are a promising new tool to probe the dynamics of individual enzymes. However, it is currently unknown whether the electrostatic signals associated with biological activity exceed detection limits. We report calculations of electrostatic signatures of two representative enzymes, deoxyribonuclease I and T4 lysozyme. Our simulations reveal that substrate binding to deoxyribonuclease and internal dynamics of lysozyme are detectable at the single-molecule level using existing point-functionalized carbon nanotube sensors.
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ISSN:1520-6106
1520-5207
DOI:10.1021/jp910946v