Single Molecule Observations of Multiple Protein Populations at the Oil−Water Interface

Single-molecule total internal reflectance fluorescence microscopy was used to observe the dynamic behavior of >4000 bovine serum albumin objects at the silicone oil−water interface. The surface residence time distribution indicated the presence of three populations at the interface. Each populat...

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Bibliographic Details
Published inLangmuir Vol. 26; no. 16; pp. 13364 - 13367
Main Authors Walder, Robert, Schwartz, Daniel K
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 17.08.2010
Subjects
Animals
Cattle
Interfaces: Adsorption, Reactions, Films, Forces
Oils - chemistry
Serum Albumin, Bovine - chemistry
Spectrometry, Fluorescence
Surface Properties
Water - chemistry
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Summary:Single-molecule total internal reflectance fluorescence microscopy was used to observe the dynamic behavior of >4000 bovine serum albumin objects at the silicone oil−water interface. The surface residence time distribution indicated the presence of three populations at the interface. Each population had a characteristic fluorescence intensity and distinctive interfacial diffusion behavior. Larger fluorescence intensity correlated with longer residence times and slower diffusion. These combined observations of fluorescence intensity, surface residence time, and interfacial diffusion suggested that the three populations represent monomers, dimers, and trimers respectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0743-7463
1520-5827
DOI:10.1021/la1023357