DNA at Aqueous/Solid Interfaces: Chirality-Based Detection via Second Harmonic Generation Activity
We present electronic spectra of single-strand and duplex DNA oligonucleotides covalently attached to fused quartz/aqueous interfaces and demonstrate that a strong nonlinear optical linear dichroism response is obtained when adenine and thymine bases undergo Watson−Crick base pairing to form a doubl...
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Published in | Journal of the American Chemical Society Vol. 131; no. 2; pp. 844 - 848 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
21.01.2009
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Subjects | |
Online Access | Get full text |
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Summary: | We present electronic spectra of single-strand and duplex DNA oligonucleotides covalently attached to fused quartz/aqueous interfaces and demonstrate that a strong nonlinear optical linear dichroism response is obtained when adenine and thymine bases undergo Watson−Crick base pairing to form a double helix. Complementary χ(3) charge screening studies indicate that the signal originates from 5 × 1011 strands per square centimeter, or 6 attomoles of surface-bound oligonucleotides. The label-free, molecular-specific nature afforded by nonlinear optical studies of DNA at aqueous/solid interfaces allows for the real-time tracking of interfacial DNA hybridization for the first time. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0002-7863 1520-5126 |
DOI: | 10.1021/ja808007b |