Exploiting Nucleotidylyltransferases To Prepare Sugar Nucleotides

Enzymatic approaches to prepare sugar nucleotides are gaining in importance and offer several advantages over chemical synthesis including high yields and stereospecificity. We report the cloning, expression, and purification of two new wild-type thymidylyltransferases and observed catalysis with a...

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Bibliographic Details
Published inOrganic letters Vol. 9; no. 5; pp. 857 - 860
Main Authors Timmons, Shannon C, Mosher, Roy H, Knowles, Sheryl A, Jakeman, David L
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 01.03.2007
Amer Chemical Soc
Subjects
Carbohydrates - chemistry
Catalysis
Chemistry
Chemistry, Organic
Molecular Structure
Nucleotides - chemistry
Nucleotides - metabolism
Nucleotidyltransferases - metabolism
Phosphates - chemistry
Physical Sciences
Science & Technology
Streptococcus - enzymology
Substrate Specificity
UDP
MECHANISM
ANALOGS
CHEMOENZYMATIC SYNTHESIS
NATURAL-PRODUCTS
GLUCOSE
BIOSYNTHETIC-PATHWAY
BIOCHEMICAL-CHARACTERIZATION
ENZYMATIC-SYNTHESIS
THYMIDYLYLTRANSFERASE
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Summary:Enzymatic approaches to prepare sugar nucleotides are gaining in importance and offer several advantages over chemical synthesis including high yields and stereospecificity. We report the cloning, expression, and purification of two new wild-type thymidylyltransferases and observed catalysis with a wide variety of substrates. Significant product inhibition was not observed with the enzymes studied over a 24 h period, enabling the efficient preparation of 15 sugar nucleotides, clearly demonstrating the synthetic utility of these biocatalysts.
Bibliography:ObjectType-Article-1
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ISSN:1523-7060
1523-7052
DOI:10.1021/ol0630853