Characterization of Gangliosides in Three Sea Urchin Species by HILIC–ESI-MS/MS

• Published in: Journal of agricultural and food chemistry Vol. 69; no. 27; pp. 7641 - 7651
• Main Authors: Ma, Yingxu, Wang, Xincen, Wang, Zhigao, Cong, Peixu, Xu, Jie, Xue, Changhu
• Format: Journal Article
• Language: English
• Published: American Chemical Society 14.07.2021
• Subjects: Bioactive Constituents, Metabolites, and Functions; semiquantitation; sulfated sialic acid; sea urchin; KDN; ganglioside
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.1c02058

Sea urchin gangliosides (SU-GLSs) are well acknowledged for their nerve regeneration activity and neuroprotective property. The present study sought to characterize and semi-quantitate different SU-GLS subclasses in three sea urchin species, including Strongylocentrotus nudus, Hemicentrotus pulcherrimus, and Glyptocidaris crenularis. A total of 14 SU-GLS subclasses were identified by a hydrophilic interaction liquid chromatography–Q-Exactive tandem mass spectrometry method. Three sialic acid (Sia) structures, including Neu5Ac, Neu5Gc, and KDN, were identified in SU-GLSs, of which Neu5Ac and Neu5Gc had their corresponding sulfated forms. The linkage among Sias was determined to be 2–8. Additionally, KDN2-6Glc1-1Cer, KDN2-8Neu5Gc2-6Glc1-1Cer, and KDN2-8Neu5Gc2-8Neu5Gc2-6Glc-1Cer were speculated to be novel SU-GLS structures. Furthermore, the total SU-GLS content was 2.0–7.3 mg/g in the three sea urchin species. These results will provide useful data for developing a SU-GLS database of aquatic products. Besides, this study will provide a theoretical basis to explore the nutritional values of seafood products further.