Label-Free, Real-Time Multiplexed DNA Detection Using Fluorescent Conjugated Polymers

A label-free, multiplexed DNA assay using fluorescent conjugated polymers as a detection probe to illustrate hybridization on metallic striped nanorods is demonstrated. Different DNA capture probes were encoded by the different reflectivities of Au and Ag stripe patterns. Successful DNA hybridizatio...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Chemical Society Vol. 131; no. 10; pp. 3432 - 3433
Main Authors Zheng, Weiming, He, Lin
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 18.03.2009
Subjects
Base Sequence
DNA - analysis
DNA Probes
Fluorescent Dyes - chemistry
Polymers - chemistry
Surface Plasmon Resonance
Online AccessGet full text

Cover

More Information
Summary:A label-free, multiplexed DNA assay using fluorescent conjugated polymers as a detection probe to illustrate hybridization on metallic striped nanorods is demonstrated. Different DNA capture probes were encoded by the different reflectivities of Au and Ag stripe patterns. Successful DNA hybridization induced an optically detectable conformational change in conjugated polythiophene derivatives from forming single-stranded DNA−polymer complexes to forming double-stranded DNA−polymer ones. The results show attomole detection sensitivity and single-mutation specificity comparable to those of single-element assays but with much improved throughput.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja809175q