2D Difference Gel Electrophoresis of Prepubertal and Pubertal Rat Mammary Gland Proteomes

Rat mammary gland proteomes at day 21 (prepubertal) and day 50 (late puberty) were compared by 2D difference gel electrophoresis. Two-hundred fifty-one spots were significantly different (p < 0.05) in abundance. Peptide mass fingerprint analysis of a subset of these proteins identified two signif...

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Published inJournal of proteome research Vol. 7; no. 11; pp. 4638 - 4650
Main Authors Kim, Helen, Cope, Mark B, Herring, Richie, Robinson, Gloria, Wilson, Landon, Page, Grier P, Barnes, Stephen
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.11.2008
Subjects
Aging
Animals
Carbocyanines - metabolism
Electrophoresis, Gel, Two-Dimensional - methods
Female
Fluorescent Dyes - metabolism
Mammary Glands, Animal - growth & development
Mammary Glands, Animal - metabolism
Peptide Mapping
Proteome - analysis
Proteome - chemistry
Proteome - genetics
Rats
Rats, Sprague-Dawley
Sexual Maturation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
2D gel
Cydye
DIGE
chemoprevention
mammary
peptide mass fingerprinting
breast cancer
puberty
false discovery rate
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Summary:Rat mammary gland proteomes at day 21 (prepubertal) and day 50 (late puberty) were compared by 2D difference gel electrophoresis. Two-hundred fifty-one spots were significantly different (p < 0.05) in abundance. Peptide mass fingerprint analysis of a subset of these proteins identified two significantly over-represented classes including structural and blood proteins (increased), and metabolism-relevant proteins (reduced) in day 50 relative to day 21 glands. This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1535-3893
1535-3907
DOI:10.1021/pr800121b