Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa Reveals Tn6352, a Novel Putative Transposon Associated with Mobilization of the blaVIM-2-Harboring In58 Integron

The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying pl...

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Published inAntimicrobial agents and chemotherapy Vol. 61; no. 5
Main Authors Botelho, João, Grosso, Filipa, Peixe, Luísa
Format Journal Article
LanguageEnglish
Published 1752 N St., N.W., Washington, DC American Society for Microbiology 01.05.2017
Subjects
Bacterial Proteins
beta-Lactamases
DNA Transposable Elements
Mechanisms of Resistance
Pseudomonas aeruginosa
transposon
Pseudomonas aeruginosa
carbapenemase
plasmid-mediated resistance
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Abstract The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying plasmids in P. aeruginosa. blaVIM-2 might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17 elements. The recognition of similar inverted repeat (IR) sites by ISPa17 reveals a common mobilization process associated with acquisition of the blaVIM-2 and blaVIM-1 genes.
AbstractList The bla VIM-2 -carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa . This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified bla VIM-2 -carrying plasmids in P. aeruginosa . bla VIM-2 might have been acquired by transposition of Tn 6352 , a novel transposon composed of the In58 and IS Pa17 elements. The recognition of similar inverted repeat (IR) sites by IS Pa17 reveals a common mobilization process associated with acquisition of the bla VIM-2 and bla VIM-1 genes.
The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying plasmids in P. aeruginosa. blaVIM-2 might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17 elements. The recognition of similar inverted repeat (IR) sites by ISPa17 reveals a common mobilization process associated with acquisition of the blaVIM-2 and blaVIM-1 genes.
Author Peixe, Luísa
Grosso, Filipa
Botelho, João
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plasmid-mediated resistance
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Citation Botelho J, Grosso F, Peixe L. 2017. Characterization of the pJB12 plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron. Antimicrob Agents Chemother 61:e02532-16. https://doi.org/10.1128/AAC.02532-16.
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Snippet The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work...
The bla VIM-2 -carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa . This work...
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SubjectTerms Bacterial Proteins
beta-Lactamases
DNA Transposable Elements
Mechanisms of Resistance
Pseudomonas aeruginosa
Title Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa Reveals Tn6352, a Novel Putative Transposon Associated with Mobilization of the blaVIM-2-Harboring In58 Integron
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