Magnetic Mesoporous Organic−Inorganic NiCo2O4 Hybrid Nanomaterials for Electrochemical Immunosensors

• Published in: ACS applied materials & interfaces Vol. 3; no. 4; pp. 1366 - 1373
• Main Authors: Li, Qunfang, Zeng, Lingxing, Wang, Jinchao, Tang, Dianping, Liu, Bingqian, Chen, Guonan, Wei, Mingdeng
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.04.2011
• Subjects: Biosensing Techniques - methods; Carcinoembryonic Antigen - analysis; Clinical Laboratory Techniques - methods; Electrochemical Techniques - methods; Horseradish Peroxidase - metabolism; Immunoassay - methods; Magnetics; Nanocomposites - chemistry; Nanopores; Sensitivity and Specificity; Staining and Labeling - methods; electrochemical immunosensor; carcinoembryonic antigen; magnetic organic−inorganic hybrid nanomaterials; mesoporous NiCo2O4 nanosheets
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am200228k

This study demonstrates a facile and feasible strategy toward the development of advanced electrochemical immunosensors based on chemically functionalized magnetic mesoporous organic−inorganic hybrid nanomaterials, and the preparation, characterization, and measurement of relevant properties of the immunosensor for detection of carcinoembryonic antigen (CEA, as a model analyte) in clinical immunoassays. The as-prepared nanomaterials composed of a magnetic mesoporous NiCo2O4 nanosheet, an interlayer of Nafion/thionine organic molecules and a nanogold layer show good adsorption properties for the attachment of horseradish peroxidase-labeled secondary anti-CEA antibody (HRP-anti-CEA). With a sandwich-type immunoassay format, the functional bionanomaterials present good analytical properties to facilitate and modulate the way it was integrated onto the electrochemical immunosensors, and allows the detection of CEA at a concentration as low as 0.5 pg/mL. Significantly, the immunosensor could be easily regenerated by only using an external magnet without the need of any dissociated reagents. Importantly, the as-synthesized magnetic mesoporous NiCo2O4 nanomaterials could be further extended for detection of other biomarkers or biocompounds.