CATCH Peptides Coassemble into Structurally Heterogeneous β‑Sheet Nanofibers with Little Preference to β‑Strand Alignment

• Published in: The journal of physical chemistry. B Vol. 125; no. 16; pp. 4004 - 4015
• Main Authors: Wong, Kong M, Shao, Qing, Wang, Yiming, Seroski, Dillon T, Liu, Renjie, Lint, Annabelle H, Hudalla, Gregory A, Hall, Carol K, Paravastu, Anant K
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 29.04.2021
• Subjects: B: Biophysical and Biochemical Systems and Processes
• ISSN: 1520-6106; 1520-5207
• DOI: 10.1021/acs.jpcb.0c11645

Coassembling peptides offer an additional degree of freedom in the design of nanostructured biomaterials when compared to analogous self-assembling peptides. Yet, our understanding of how amino acid sequences encodes coassembled nanofiber structure is limited. Prior work on a charge-complementary pair, CATCH+ and CATCH– peptides, detected like-peptide nearest neighbors (CATCH+:CATCH+ and CATCH–:CATCH−) within coassembled β-sheet nanofibers; these self-associated peptide pairs marked a departure from an “ideal” coassembled structure. In this work, we employ solid-state NMR, isotope-edited FTIR, and coarse-grained molecular dynamics simulations to evaluate the alignment of β-strands within CATCH peptide nanofibers. Both experimental and computational results suggest that CATCH molecules coassemble into structurally heterogeneous nanofibers, which is consistent with our observations in another coassembling system, the King–Webb peptides. Within β-sheet nanofibers, β-strands were found to have nearest neighbors aligned in-register parallel, in-register antiparallel, and out-of-register. In comparison to the King-Webb peptides, CATCH nanofibers exhibit a greater degree of structural heterogeneity. By comparing the amino acid sequences of CATCH and King–Webb peptides, we can begin to unravel sequence-to-structure relationships, which may encode more precise coassembled β-sheet nanostructures.