Heterogeneity of rat tropoelastin mRNA revealed by cDNA cloning

• Published in: Biochemistry (Easton) Vol. 29; no. 41; pp. 9677 - 9683
• Main Authors: Pierce, Richard A, Deak, Susan B, Stolle, Catherine A, Boyd, Charles D
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.10.1990
• Subjects: 550201 - Biochemistry- Tracer Techniques; AMINO ACID SEQUENCE; ANIMAL TISSUES; Animals; Animals, Newborn; AORTA; Aorta - metabolism; ARTERIES; Base Sequence; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; Cattle; CLONING; Cloning, Molecular - methods; CONNECTIVE TISSUE; DAYS LIVING RADIOISOTOPES; DNA; DNA - genetics; DNA HYBRIDIZATION; DNA SEQUENCING; DNA-CLONING; Gene Library; HYBRIDIZATION; ISOTOPES; LIGHT NUCLEI; MESSENGER-RNA; Molecular Sequence Data; MOLECULAR STRUCTURE; Muscle, Smooth, Vascular - metabolism; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS 32; PHOSPHORUS ISOTOPES; Poly A - genetics; PROTEINS; RADIOISOTOPES; Rats; RECOMBINANT DNA; Restriction Mapping; RNA; RNA - genetics; RNA, Messenger - genetics; Sequence Homology, Nucleic Acid; STRUCTURAL CHEMICAL ANALYSIS; TISSUES; Tropoelastin - genetics
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00493a024

A lambda gt11 library constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats was screened for rat tropoelastin cDNAs. The first screen, utilizing a human tropoelastin cDNA clone, provided rat tropoelastin cDNAs spanning 2.3 kb of carboxy-terminal coding sequence and extended into the 3'-untranslated region. A subsequent screen using a 5' rat tropoelastin cDNA clone yielded clones extending into the amino-terminal signal sequence coding region. Sequence analysis of these clones has provided the complete derived amino acid sequence of rat tropoelastin and allowed alignment and comparison with published bovine cDNA sequence. While the overall structure of rat tropoelastin is similar to bovine sequence, numerous substitutions, deletions, and insertions demonstrated considerable heterogeneity between species. In particular, the pentapeptide repeat VPGVG, characteristic of all tropoelastins analyzed to date, is replaced in rat tropoelastin by a repeating pentapeptide, IPGVG. The hexapeptide repeat VGVAPG, the bovine elastin receptor binding peptide, is not encoded by rat tropoelastin cDNAs. Variations in coding sequence between rat tropoelastin cDNA clones were also found which may represent mRNA heterogeneity produced by alternative splicing of the rat tropoelastin pre-mRNA.