A Series of Ferrocene-Containing Pyrazolo[1,5‑a]pyrimidines Induce a Strong Antiproliferative Effect against Oral Cancer Cells

• Published in: Organometallics Vol. 41; no. 16; pp. 2365 - 2378
• Main Authors: Gupta, Astha, Das, Rudradip, Chamoli, Ambika, Choithramani, Asmita, Kumar, Hansal, Patel, Sagarkumar, Khude, Datta, Bothra, Gourav, Wangdale, Khushal, Ghosh Chowdhury, Moumita, Rathod, Rajeshwari, Mandoli, Amit, Shard, Amit
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 22.08.2022; Amer Chemical Soc
• Subjects: Chemistry; Chemistry, Inorganic & Nuclear; Chemistry, Organic; Physical Sciences; Science & Technology; PYRAZOLE; DESIGN; PROTOCOLS; ESTROGEN-RECEPTOR MODULATORS; DRUGS; REDOX; INHIBITOR; DERIVATIVES; BREAST; RANGE
• ISSN: 0276-7333; 1520-6041
• DOI: 10.1021/acs.organomet.2c00348

Bio-organometallic agents confer superior physicochemical profiles inaccessible to purely organic molecules. As current chemotherapeutic regimes are maligned by the toxicity and chemoresistance of cisplatin and carboplatin, the development of an arsenal of metallodrugs becomes indispensable. Metallocenes such as ferrociphenols, JAHA, and ferrocene-labeled estradiol exhibit potent anticancer profiles. Oral squamous cell carcinomas are shown to be an excellent model to assess the impact of ferrocene-based pyrazolopyrimidines (FPPs) as chemotherapeutic agents. We show here synthesis and preliminary mechanistic investigations of FPPs in the CAL 27 cell line. The results established that some of the FPPs like 4u display strong antiproliferative behavior selectively against cisplatin-resistant oral cancer cells with an IC50 value of 7.49 μM. It was also found to activate tumor pyruvate kinase M2 (PKM2), a critical metabolic conduit in cancer. Furthermore, 4u induced apoptosis in cells and arrested cell growth in the S-phase of the cell cycle. In silico studies established that the molecule binds at the DASA-58 binding site of PKM2.