Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles

• Published in: The journal of physical chemistry. B Vol. 106; no. 34; pp. 8493 - 8498
• Main Authors: Cheng, Ji-xin, Volkmer, Andreas, Book, Lewis D, Xie, X. Sunney
• Format: Journal Article
• Language: English
• Published: American Chemical Society 29.08.2002
• ISSN: 1520-6106; 1520-5207
• DOI: 10.1021/jp025771z

We report a theoretical description and experimental implementation of multiplex coherent anti-Stokes Raman scattering (M-CARS) microspectroscopy using a picosecond pump beam and a femtosecond Stokes beam. The effect of the chirp in the Stokes pulse on a M-CARS spectrum is studied. Polarization-sensitive detection is utilized for suppression of the nonresonant background and for selective detection of spectrally overlapped Raman bands. M-CARS spectra of liposomes in the C−H stretching vibration region were acquired. Polarization CARS imaging of lipids in live cells based on the M-CARS spectra is demonstrated.