Designed Signaling Aptamers that Transduce Molecular Recognition to Changes in Fluorescence Intensity

We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution. Two different anti-adenosine “signaling aptamers”, one made from RNA and one from DNA, can selectively signal the presence of adenosine in solution. Increases in fluorescence i...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 122; no. 11; pp. 2469 - 2473
Main Authors Jhaveri, Sulay D, Kirby, Romy, Conrad, Rick, Maglott, Emily J, Bowser, Michael, Kennedy, Robert T, Glick, Gary, Ellington, Andrew D
Format Journal Article
LanguageEnglish
Published American Chemical Society 22.03.2000
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Summary:We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution. Two different anti-adenosine “signaling aptamers”, one made from RNA and one from DNA, can selectively signal the presence of adenosine in solution. Increases in fluorescence intensity reproducibly follow increases in adenosine concentration, and can be used for quantitation. The facile methods we have developed can potentially be used for generating a wide variety of signaling aptamers for use in sensor arrays.
Bibliography:ark:/67375/TPS-LM42VRL0-1
istex:B123006D39D399CC4913A4B1BD12573518754DB3
ISSN:0002-7863
1520-5126
DOI:10.1021/ja992393b