Development of the Au@Pt-Labeled Nanobody Lateral-Flow Nanozyme Immunoassay for Visual Detection of 3‑Phenoxybenzoic Acid in Milk and Lake Water

As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. A gold–platinum (Au@Pt) nanozyme is a kind of mimic enzyme with unique properties of nanomaterials and catalytic activity. The anti-3-PBA nanobodies were la...

Full description

Saved in:
Bibliographic Details
Published inACS agricultural science & technology Vol. 2; no. 3; pp. 573 - 579
Main Authors Zhang, Can, Hu, Jinnuo, Wu, Xiaoxiao, Shi, Jiyong, Hammock, Bruce D
Format Journal Article
LanguageEnglish
Published American Chemical Society 20.06.2022
Subjects
Au@Pt nanozymes
nanobodies
3-phenoxybenzoic acid
lateral-flow immunoassay
Online AccessGet full text

Cover

More Information
Summary:As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. A gold–platinum (Au@Pt) nanozyme is a kind of mimic enzyme with unique properties of nanomaterials and catalytic activity. The anti-3-PBA nanobodies were labeled with Au@Pt (Au@Pt-Nbs) by electrostatic adsorption. The Au@Pt-Nbs lateral-flow nanozyme immunoassay was established for the visual and rapid detection of 3-PBA. The visualization results were quantitatively analyzed by Adobe Photoshop CC and got a cutoff value of 0.005 ng/mL. The samples of milk- and lake water-spiked 3-PBA were also analyzed, and the recoveries ranged from 102 to 108% and 95 to 112%, respectively. The results were also validated by liquid chromatography–mass spectrometry and got good correlation (R 2 = 0.945). The Au@Pt-Nbs lateral-flow nanozyme immunoassay was developed by the combination of nanobodies and nanozymes, which will be a promising application in the field of safety inspection.
ISSN:2692-1952
2692-1952
DOI:10.1021/acsagscitech.2c00018