Negative Magnetophoresis Focusing Microchips Online-Coupled with ICP–MS for High-Throughput Single-Cell Analysis
High-throughput single-cell analysis is critical to elucidate the cell heterogeneity. Recently, droplet microchips using oil/gas phases to generate single-cell encapsulated droplets have been combined with inductively coupled plasma–mass spectrometry (ICP–MS) for determination of trace elements in s...
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Published in | Analytical chemistry (Washington) Vol. 94; no. 18; pp. 6649 - 6656 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
10.05.2022
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Subjects | |
Online Access | Get full text |
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Summary: | High-throughput single-cell analysis is critical to elucidate the cell heterogeneity. Recently, droplet microchips using oil/gas phases to generate single-cell encapsulated droplets have been combined with inductively coupled plasma–mass spectrometry (ICP–MS) for determination of trace elements in single cells with a throughput of dozens of cells per min. To improve the sample throughput and avoid the oil phase introduced into ICP–MS, herein, a negative magnetophoresis focusing microchip was established and online-coupled to ICP–MS for single-cell analysis. MCF-7 cells in the paramagnetic salt solution were introduced into the designed focusing microchannel, in which they were focused into a single stream under both the magnetic repulsion force and inertial lift force, and then were introduced into ICP–MS for online single-cell analysis. The important parameters including the chip design, the concentration of the paramagnetic salt solution, flow rate, cell density, and dwell time were optimized. Under the optimal conditions, a high sample throughput of 1390 cells min–1 was obtained. The established online analytical system was applied to study the uptake behaviors of MCF-7 cells for Zn2+ and ZnO nanoparticles (NPs) at a single-cell level. The single-cell analysis results indicate that MCF-7 cells displayed more remarkable heterogeneity when they were treated with ZnO NPs, and the uptake content of ZnO NPs by MCF-7 cells was less than that of Zn2+. Compared with other droplet microdevice-ICP–MS analysis systems, the developed system has the advantages of simple design and fabrication, no organic phase, a high throughput, and a low sample consumption (only 5 μL). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/acs.analchem.1c04216 |