Duplex-Specific Nuclease-Enabled Target Recycling on Semiconducting Metal–Organic Framework Heterojunctions for Energy-Transfer-Based Organic Photoelectrochemical Transistor miRNA Biosensing

Semiconductor metal–organic frameworks (MOFs) and heterojunctions have gained increasing attention in many fields, yet their full potential remains largely unexplored. Advanced optobioelectronics are envisioned to create more opportunities for innovative biomedical applications. This study reports a...

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Published inAnalytical chemistry (Washington) Vol. 94; no. 45; pp. 15856 - 15863
Main Authors Gao, Ge, Chen, Jia-Hao, Li, Cheng-Jun, Wang, Cheng-Shuang, Hu, Jin, Zhou, Hong, Lin, Peng, Xu, Qin, Zhao, Wei-Wei
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 15.11.2022
Subjects
Biomedical materials
Biosensing Techniques
Biosensors
Cadmium sulfide
Channel gating
Chemistry
Electrochemical Techniques
Endonucleases
Energy Transfer
Heterojunctions
Hybridization
Limit of Detection
Medical innovations
Metal Nanoparticles
Metal-Organic Frameworks
MicroRNAs
MicroRNAs - genetics
miRNA
Nuclease
Polystyrene resins
Quantum Dots
Recycling
Ribonucleic acid
RNA
Semiconductor devices
Styrene
Transistors
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Summary:Semiconductor metal–organic frameworks (MOFs) and heterojunctions have gained increasing attention in many fields, yet their full potential remains largely unexplored. Advanced optobioelectronics are envisioned to create more opportunities for innovative biomedical applications. This study reports a UiO-66-NH2 (U6N)/CdS quantum dots (QDs)-gated organic photoelectrochemical transistor (OPECT) and its application toward energy-transfer-based sensitive microRNA-166a (miRNA-166a) detection assisted by duplex-specific nuclease (DSN)-enabled target recycling. Specifically, a U6N/CdS QDs photoanode was fabricated and shown to be efficiently gating a poly­(3,4-ethylenedioxythiophene) doped with poly­(styrene sulfonate) (PEDOT/PSS) channel, while the DSN-enabled release of Au-reporters and hybridization upon the U6N/CdS QDs photoanode could significantly inhibit the photoanode response via an energy transfer process and thus modulate the device response, permitting novel dual-amplified optobioelectronic miRNA-166a detection with a low detection limit of 1.0 fM. This work not only features the DSN-amplified miRNA detection via an OPECT route but also unveils the potential of semiconductor MOF heterojunctions for futuristic optobioelectronics.
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ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.2c03859