Electrophoretic Deposition of 58S Bioactive Glass- Polymer Composite Coatings on 316L Stainless Steel: An Optimization for Corrosion, Bioactivity, and Cytocompatibility

• Published in: ACS applied bio materials Vol. 7; no. 5; pp. 2966 - 2981
• Main Authors: Hadem, Hushnaara, Mitra, Arijit, Ojha, Atul Kumar, Rajasekaran, Ragavi, Satpathy, Bangmaya, Das, Debasish, Mukherjee, Sayan, Dhara, Santanu, Das, Siddhartha, Das, Karabi
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.05.2024
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Cell Proliferation - drug effects; Cell Survival - drug effects; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Corrosion; Electrophoresis; Escherichia coli - drug effects; Glass - chemistry; Humans; Materials Testing; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Microbial Sensitivity Tests; Particle Size; Polymers - chemistry; Polymers - pharmacology; Stainless Steel - chemistry; Staphylococcus aureus - drug effects; Surface Properties; cytocompatibility; electrophoretic deposition; antibacterial; 58S bioactive glass; bioactive glass-polymer composite coating
• ISSN: 2576-6422; 2576-6422
• DOI: 10.1021/acsabm.4c00037

This study presents a facile fabrication of 58S bioactive glass (BG)-polymer composite coatings on a 316L stainless steel (SS) substrate using the electrophoretic deposition technique. The suspension characteristics and deposition kinetics of BG, along with three different polymers, namely ethylcellulose (EC), poly­(acrylic acid) (PAA), and polyvinylpyrrolidone (PVP), have been utilized to fabricate the coatings. Among all coatings, 58S BG and EC polymers are selected as the final composite coating (EC6) owing to their homogeneity and good adhesion. EC6 coating exhibits a thickness of ∼18 μm and an average roughness of ∼2.5 μm. Herein, EC6 demonstrates better hydroxyapatite formation compared to PAA and PVP coatings in simulated body fluid-based mineralization studies for a period of 28 days. Corrosion studies of EC6 in phosphate-buffered saline further confirm the higher corrosion resistance properties after 14 days. In vitro cytocompatibility studies using human placental mesenchymal stem cells demonstrate an increase in cellular viability, attachment, and higher proliferation compared to the bare SS substrate. EC6 coatings promote osteogenic differentiation, which is confirmed via the upregulation of the OPN and OCN genes. Moreover, the EC6 sample exhibits improved antibacterial properties against Escherichia coli and Staphylococcus aureus compared to the uncoated ones. The findings of this work emphasize the potential of electrophoretically fabricated BG–EC composite coatings on SS substrates for orthopedic applications.