Nonphotochemical Laser Induced Nucleation of Hen Egg White Lysozyme Crystals

Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were...

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Bibliographic Details
Published inCrystal growth & design Vol. 8; no. 12; pp. 4255 - 4261
Main Authors Lee, In Sung, Evans, James M. B, Erdemir, Deniz, Lee, Alfred Y, Garetz, Bruce A, Myerson, Allan S
Format Journal Article Conference Proceeding
LanguageEnglish
Published Washington,DC American Chemical Society 03.12.2008
Subjects
Condensed matter: structure, mechanical and thermal properties
Equations of state, phase equilibria, and phase transitions
Exact sciences and technology
General studies of phase transitions
Nucleation
Physics
Proteins
Radiation effects
Nucleation
Laser pulse
Macromolecules
Droplets
Egg white
Laser irradiation
Lysozyme
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Summary:Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were observed in a given time period, compared with nonirradiated samples. As a general rule, nonphotochemical laser induced nucleation (NPLIN) in lysozyme solutions was more effective with shorter aging time, 532-nm wavelength, higher peak intensity, and shorter pulse duration. Bovine pancreatic trypsin (BPT) was also examined using NPLIN, showing the potential application of NPLIN to other proteins.
ISSN:1528-7483
1528-7505
DOI:10.1021/cg800696u