キモパパインによる犬椎間板の実験的Chemonucleolysis
犬の椎間板内へのキモパパイン注入により生ずるChemonucleolysisの経過をX線学的ならびに組織学的に観察した. キモパパイン注入によりX線学的に狭小化を示した椎間板腔は2週目より徐々に回復を示し, 12週目には0.1mg例で74.1%, 1.0mg例で61.1%および10.0mg例で71.7%まで回復していた. また, 加齢により回復が遅延する傾向がみられた. 組織学的には, キモパパイン注入2週目には椎間板腔が減少を示し, 投与酵素量に応じてプロテオグリカン陽性基質が減少していた. 髄核は増殖軟骨細胞とコラーゲン基質で囲環された不整形の変性脊索細胞塊より成っていた. 12週目には,...
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| Published in | Journal of Veterinary Medical Science Vol. 55; no. 2; pp. 211 - 215 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
公益社団法人 日本獣医学会
1993
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| Online Access | Get full text |
| ISSN | 0916-7250 1347-7439 |
| DOI | 10.1292/jvms.55.211 |
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| Abstract | 犬の椎間板内へのキモパパイン注入により生ずるChemonucleolysisの経過をX線学的ならびに組織学的に観察した. キモパパイン注入によりX線学的に狭小化を示した椎間板腔は2週目より徐々に回復を示し, 12週目には0.1mg例で74.1%, 1.0mg例で61.1%および10.0mg例で71.7%まで回復していた. また, 加齢により回復が遅延する傾向がみられた. 組織学的には, キモパパイン注入2週目には椎間板腔が減少を示し, 投与酵素量に応じてプロテオグリカン陽性基質が減少していた. 髄核は増殖軟骨細胞とコラーゲン基質で囲環された不整形の変性脊索細胞塊より成っていた. 12週目には, 髄核中心は線維軟骨組織に置換されていた. 10.0mg例では椎間板腔は変性基質を伴わない密な軟骨組織で満たされ, 椎間板腔が狭小化していた. これらの結果より, キモパパイン10.0mgによるChemonucleolysisは化学的椎間板減圧術として犬の椎間板内圧を減少させ, 椎間板ヘルニアの病状を軽減すると考えられる. |
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| AbstractList | 犬の椎間板内へのキモパパイン注入により生ずるChemonucleolysisの経過をX線学的ならびに組織学的に観察した. キモパパイン注入によりX線学的に狭小化を示した椎間板腔は2週目より徐々に回復を示し, 12週目には0.1mg例で74.1%, 1.0mg例で61.1%および10.0mg例で71.7%まで回復していた. また, 加齢により回復が遅延する傾向がみられた. 組織学的には, キモパパイン注入2週目には椎間板腔が減少を示し, 投与酵素量に応じてプロテオグリカン陽性基質が減少していた. 髄核は増殖軟骨細胞とコラーゲン基質で囲環された不整形の変性脊索細胞塊より成っていた. 12週目には, 髄核中心は線維軟骨組織に置換されていた. 10.0mg例では椎間板腔は変性基質を伴わない密な軟骨組織で満たされ, 椎間板腔が狭小化していた. これらの結果より, キモパパイン10.0mgによるChemonucleolysisは化学的椎間板減圧術として犬の椎間板内圧を減少させ, 椎間板ヘルニアの病状を軽減すると考えられる. |
| Author | 橋本, 晃 角, 明美 工藤, 忠明 |
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| References | 4. Gage, E. D. 1972. Spinal disc surgery in the dog I. Cervical disc fenestration. Southwestern Vet. 25: 21-30. 13. Saunders, E. C. 1964. Treatment of the canine intervertebral disk syndrome with chymopapain. J. Am. Vet. Med. Assoc. 145: 893-896. 7. Gravin, P. J., Jennings, R. B., Smith, L., and Gesler, R. M. 1965. Chymopapain: A pharmacologic and toxicologic evaluation in experimental animals. Clin. Orthop. 42: 204-223. 8. Gravin, P. J. and Jinnings, R. B. 1973. Long-term effects of chymopapain on intervertebral disks of dogs. Clin. Orthop. 92: 281-295. 1. Atilola, M. A. O., Morgan, J. P., Bailey, C. S., and Miyabayashi, T. 1988. Canine chemonucleolysis: An experimenal radiographic study. Vet. Radiol. 29: 168-175. 2. Bradford, D. S., Cooper, K. M., and Oegema, T. R. 1983. Chymopapain chemonucleolysis, and nucleus pulposus regeneration. J. Bone Jt. Surg., Am. Vol. 65A: 1220-1231. 11. Hirsch, C. 1959. Studies on the pathology of low back pain. J. Bone Jt. Surg., Br. Vol. 49: 237-243. 10. Hayashi, K. 1991. Histological study on chemonucleolysis with special reference to the relationship between the dose of chymopapain and disk regeneration. J. Jpn. Orthop. Assoc. 65: 216-229. 12. Pettit, G. D. 1966. Intervertebral disc protrusion in the dog. Appleton-Century-Crofts, New York. 16. Takahashi, K., Inoue, S., Takada, S., Nishiyama, H., Mimura, M., and Wada, Y. 1986. Experimental study on chemonucleolysis with special reference to the change of intradiskal pressure. Spine 11: 617-620. 5. Gage, E. D. 1972. Spinal disc surgery in the dog II. Cervical laminectomy. Southwestern Vet. 25: 115-119. 6. Gage, E. D. 1972. Spinal disc surgery in the dog III. Thoracolumbar hemilaminectomy and disc fenestration. Southwestern Vet. 25: 209-215. 15. Smith, L. and Brown, J. E. 1967. Treatment of lumbar intervertebral disk lesions by direct injection of chymopapain. J. Bone Jt. Surg., Br. Vol., 41: 502-519. 3. Bradford, D. S., Oegema, T. R., Cooper, K. M., and Chao, E. Y. 1984. Chymopapain chemonucleolysis, and nucleus pulposus regeneration. A biochemical and biomechanical study. Spine 9: 135-147. 14. Smith, L. 1964. Enzyme dissolution of the nucleus pulposus in humans. J. Am. Med. Assoc. 187: 137-149. 9. Hansen, H-J. 1951. A pathologic-anatomical interpretation of disk degeneration in dogs. Acta Orthop. Scand. 20: 280-293. |
| References_xml | – reference: 16. Takahashi, K., Inoue, S., Takada, S., Nishiyama, H., Mimura, M., and Wada, Y. 1986. Experimental study on chemonucleolysis with special reference to the change of intradiskal pressure. Spine 11: 617-620. – reference: 13. Saunders, E. C. 1964. Treatment of the canine intervertebral disk syndrome with chymopapain. J. Am. Vet. Med. Assoc. 145: 893-896. – reference: 10. Hayashi, K. 1991. Histological study on chemonucleolysis with special reference to the relationship between the dose of chymopapain and disk regeneration. J. Jpn. Orthop. Assoc. 65: 216-229. – reference: 1. Atilola, M. A. O., Morgan, J. P., Bailey, C. S., and Miyabayashi, T. 1988. Canine chemonucleolysis: An experimenal radiographic study. Vet. Radiol. 29: 168-175. – reference: 3. Bradford, D. S., Oegema, T. R., Cooper, K. M., and Chao, E. Y. 1984. Chymopapain chemonucleolysis, and nucleus pulposus regeneration. A biochemical and biomechanical study. Spine 9: 135-147. – reference: 15. Smith, L. and Brown, J. E. 1967. Treatment of lumbar intervertebral disk lesions by direct injection of chymopapain. J. Bone Jt. Surg., Br. Vol., 41: 502-519. – reference: 9. Hansen, H-J. 1951. A pathologic-anatomical interpretation of disk degeneration in dogs. Acta Orthop. Scand. 20: 280-293. – reference: 6. Gage, E. D. 1972. Spinal disc surgery in the dog III. Thoracolumbar hemilaminectomy and disc fenestration. Southwestern Vet. 25: 209-215. – reference: 8. Gravin, P. J. and Jinnings, R. B. 1973. Long-term effects of chymopapain on intervertebral disks of dogs. Clin. Orthop. 92: 281-295. – reference: 14. Smith, L. 1964. Enzyme dissolution of the nucleus pulposus in humans. J. Am. Med. Assoc. 187: 137-149. – reference: 2. Bradford, D. S., Cooper, K. M., and Oegema, T. R. 1983. Chymopapain chemonucleolysis, and nucleus pulposus regeneration. J. Bone Jt. Surg., Am. Vol. 65A: 1220-1231. – reference: 7. Gravin, P. J., Jennings, R. B., Smith, L., and Gesler, R. M. 1965. Chymopapain: A pharmacologic and toxicologic evaluation in experimental animals. Clin. Orthop. 42: 204-223. – reference: 5. Gage, E. D. 1972. Spinal disc surgery in the dog II. Cervical laminectomy. Southwestern Vet. 25: 115-119. – reference: 4. Gage, E. D. 1972. Spinal disc surgery in the dog I. Cervical disc fenestration. Southwestern Vet. 25: 21-30. – reference: 11. Hirsch, C. 1959. Studies on the pathology of low back pain. J. Bone Jt. Surg., Br. Vol. 49: 237-243. – reference: 12. Pettit, G. D. 1966. Intervertebral disc protrusion in the dog. Appleton-Century-Crofts, New York. |
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| Snippet | 犬の椎間板内へのキモパパイン注入により生ずるChemonucleolysisの経過をX線学的ならびに組織学的に観察した. キモパパイン注入によりX線学的に狭小化を示した椎間板腔は2週目より徐々に回復を示し, 12週目には0.1mg例で74.1%,... |
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| Title | キモパパインによる犬椎間板の実験的Chemonucleolysis |
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