オウム病クラミジアの増殖と各種抗原物質の出現時期に関する研究
Chlamydis psittaci(C. psittaci)のIzawa-1株とP-1041株をそれぞれVero E 6細胞に接種し, 単クローン性抗体を用いた間接蛍光抗体法により経時的に感染細胞におけるクラミジア抗原の産生を検討した. 属特異的なリポ多糖類抗原は全増殖過程を通じて検出された. 一方, 蛋白抗原において, 各種単クローン性抗体で検出される抗原決定基は感染後一定の時期を経て検出されるものから全増殖過程を通じて検出されるものまで多様であった. また両株に交差反応を示す各種単クローン性抗体を用いて, 共通抗原の出現時期を検討したところ, その出現時期は株によって異なり, ホモ株でヘ...
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| Published in | Journal of Veterinary Medical Science Vol. 53; no. 4; pp. 691 - 697 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
公益社団法人 日本獣医学会
1991
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| Online Access | Get full text |
| ISSN | 0916-7250 1347-7439 |
| DOI | 10.1292/jvms.53.691 |
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| Abstract | Chlamydis psittaci(C. psittaci)のIzawa-1株とP-1041株をそれぞれVero E 6細胞に接種し, 単クローン性抗体を用いた間接蛍光抗体法により経時的に感染細胞におけるクラミジア抗原の産生を検討した. 属特異的なリポ多糖類抗原は全増殖過程を通じて検出された. 一方, 蛋白抗原において, 各種単クローン性抗体で検出される抗原決定基は感染後一定の時期を経て検出されるものから全増殖過程を通じて検出されるものまで多様であった. また両株に交差反応を示す各種単クローン性抗体を用いて, 共通抗原の出現時期を検討したところ, その出現時期は株によって異なり, ホモ株でヘテロ株より早いものからヘテロ株でホモ株より早いものまで変化がみられた. 次に電子顕微鏡により細胞質封入体内に含まれるクラミジア粒子の経時的な形態変化を観察し, 単クローン性抗体による特異抗原の出現との関連を検討した結果, C.psittaciの蛋白抗原上の抗原決定基は基本小体と網様体に共通なもの, および基本小体に特異的なものの2群に分類された. |
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| AbstractList | Chlamydis psittaci(C. psittaci)のIzawa-1株とP-1041株をそれぞれVero E 6細胞に接種し, 単クローン性抗体を用いた間接蛍光抗体法により経時的に感染細胞におけるクラミジア抗原の産生を検討した. 属特異的なリポ多糖類抗原は全増殖過程を通じて検出された. 一方, 蛋白抗原において, 各種単クローン性抗体で検出される抗原決定基は感染後一定の時期を経て検出されるものから全増殖過程を通じて検出されるものまで多様であった. また両株に交差反応を示す各種単クローン性抗体を用いて, 共通抗原の出現時期を検討したところ, その出現時期は株によって異なり, ホモ株でヘテロ株より早いものからヘテロ株でホモ株より早いものまで変化がみられた. 次に電子顕微鏡により細胞質封入体内に含まれるクラミジア粒子の経時的な形態変化を観察し, 単クローン性抗体による特異抗原の出現との関連を検討した結果, C.psittaciの蛋白抗原上の抗原決定基は基本小体と網様体に共通なもの, および基本小体に特異的なものの2群に分類された. |
| Author | 橋本, 信夫 諏訪, 隆彦 安藤, 秀二 高島, 郁夫 |
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| References | 17. Reeve, P. and Taverne, J. 1962. Some properties of the complement-fixing antigens of the agents of trachoma and inclusion blennorrhoea and the relationship of the antigens to the developmental cycle. J. Gen. Microbiol. 27: 501-508. 8. Hatch, T. P., Vance, D. W. Jr., and Al-Hossainy, E. 1981. Identification of a major envelope protein in Chlamydia spp. J. Bacteriol. 146: 426-429. 16. Peeling, R., Maclean, I. W., and Brunhan, R. C. 1984. In vitro neutralization of Chlamydia trachomatis with monoclonal antibody to an epitope on the major outer membrane protein. Infect. Immun. 46: 484-488. 22. Wang, S. -P., Kuo, C. -C., and Grayston, J. T. 1973. A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms. Infect. Immun. 7: 356-360. 20. Takahashi, T., Takashima, I., and Hashimoto, N. 1988. Immunotyping of Chlamydia psittaci by indirect immunofluorescence antibody test with monoclonal antiboides. Microbiol. Immunol. 32: 251-263. 3. Cevenini, R., Donati, M., Sambri, V., Rumpianesi, F., and La Placa, M. 1987. Reactivity of elementary and reticulate bodies of Chlamydia trachomatis LGV2 with monoclonal antibodies specific for the major outer membrane protein. FEMS Microbiol. Lett. 42: 47-51. 10. Higashi, N. 1964. The mode of reproduction of the psittacosis-lymphogranuloma-trachoma (PLT) group viruses. Int. Rev. Exp. Pathol. 3: 35-64. 1. Bavoil, P., Ohlin, A., and Schachter, J. 1984. Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis. Infect. Immun. 44: 479-485. 7. Hatch, T. P., Miceli, M., and Sublett, J. E. 1986. Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of Chlamydia psittaci and Chlamydia trachomatis. J. Bacteriol. 165: 379-385. 13. Matsumoto, A., Bessho, H., Soejima, R., and Hino, J. 1984. Biological properties of a Chlamydia strain isolated from a pet bird, budgerigar which was kept by a psittacosis patient. Kawasaki Med. J. 10: 77-90. 18. Richmond, S. J. and Stirling, P. 1981. Localization of chlamydial group antigen in McCoy cell monolayers infected with Chlamydia trachomatis or Chlamydia psittaci. Infect. Immun. 34: 561-570. 11. Higashi, N. 1965. Electron microscopic studies on the mode of reproduction of trachoma virus and psittacosis virus in cell cultures. Exp. Mol. Pathol. 4: 24-39. 6. Hackstadt, T., Todd, W. J., and Caldwell, H. D. 1985. Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of Chalmydiae? J. Bacteriol. 161: 25-31. 5. Dhir, S. P. and Boatman, E. S. 1972. Location of polysaccharide on Chlamydia psittaci by silver-methenamine staining and electron microscopy. J. Bacteriol. 111: 267-271. 2. Birkelund, S., Lundemose, A. G., and Christiansen, G. 1989. Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis. Infect. Immun. 57: 3250-3253. 9. Higashi, N., Tamura, A., and Iwanaga, M. 1962. Developmental cycle and reproductive mechanism of the meningopneumonitis virus in strain L cells. Ann. New York Acad. Sci. 98: 100-121. 4. Chiba, N., Arikawa, J., Takashima, I., and Hashimoto, N. 1984. Isolation and serological survey of chlamydiosis in feral pigeons and crows in Hokkaido. Jpn. J. Vet. Sci. 46: 243-245. 14. Moulder, J. W., Hatch, T. P., Kuo, C. -C., Schachter, J., and Storz, J. 1984. Genus Chlamydia. pp. 729-739. In: Bergey's Manual of Systematic Bacteriology, vol. 1 (Krieg, N. R. and Holt, J. G. eds.), The Williams & Wilkins Co., Baltimore. 12. Karimi, S. T., Schloemer, R. H., and Wilde III, C. E. 1989. Accumulation of chlamydial lipopolysaccharide antigen in the plasma membranes of infected cells. Infect. Immun. 57: 1780-1785. 15. Newhall, V. W. J. 1987. Biosynthesis and disulfide crosslinking of outer membrane components during the growth cycle of Chlamydia trachomatis. Infect. Immun. 55: 162-168. 19. Seki, C., Takashima, I., Arikawa, J., and Hashimoto, N. 1988. Monoclonal antibodies to Chlamydia psittaci: characteristics and antigenic analysis. Jpn. J. Vet. Sci. 50: 383-393. 21. Toyofuku, H., Takashima, I., Arikawa, J., and Hashimoto, N. 1986. Monoclonal antibodies against Chlamydia psittaci. Microibol. Immunol. 30: 945-955. |
| References_xml | – reference: 9. Higashi, N., Tamura, A., and Iwanaga, M. 1962. Developmental cycle and reproductive mechanism of the meningopneumonitis virus in strain L cells. Ann. New York Acad. Sci. 98: 100-121. – reference: 11. Higashi, N. 1965. Electron microscopic studies on the mode of reproduction of trachoma virus and psittacosis virus in cell cultures. Exp. Mol. Pathol. 4: 24-39. – reference: 4. Chiba, N., Arikawa, J., Takashima, I., and Hashimoto, N. 1984. Isolation and serological survey of chlamydiosis in feral pigeons and crows in Hokkaido. Jpn. J. Vet. Sci. 46: 243-245. – reference: 8. Hatch, T. P., Vance, D. W. Jr., and Al-Hossainy, E. 1981. Identification of a major envelope protein in Chlamydia spp. J. Bacteriol. 146: 426-429. – reference: 18. Richmond, S. J. and Stirling, P. 1981. Localization of chlamydial group antigen in McCoy cell monolayers infected with Chlamydia trachomatis or Chlamydia psittaci. Infect. Immun. 34: 561-570. – reference: 21. Toyofuku, H., Takashima, I., Arikawa, J., and Hashimoto, N. 1986. Monoclonal antibodies against Chlamydia psittaci. Microibol. Immunol. 30: 945-955. – reference: 10. Higashi, N. 1964. The mode of reproduction of the psittacosis-lymphogranuloma-trachoma (PLT) group viruses. Int. Rev. Exp. Pathol. 3: 35-64. – reference: 2. Birkelund, S., Lundemose, A. G., and Christiansen, G. 1989. Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis. Infect. Immun. 57: 3250-3253. – reference: 15. Newhall, V. W. J. 1987. Biosynthesis and disulfide crosslinking of outer membrane components during the growth cycle of Chlamydia trachomatis. Infect. Immun. 55: 162-168. – reference: 19. Seki, C., Takashima, I., Arikawa, J., and Hashimoto, N. 1988. Monoclonal antibodies to Chlamydia psittaci: characteristics and antigenic analysis. Jpn. J. Vet. Sci. 50: 383-393. – reference: 14. Moulder, J. W., Hatch, T. P., Kuo, C. -C., Schachter, J., and Storz, J. 1984. Genus Chlamydia. pp. 729-739. In: Bergey's Manual of Systematic Bacteriology, vol. 1 (Krieg, N. R. and Holt, J. G. eds.), The Williams & Wilkins Co., Baltimore. – reference: 17. Reeve, P. and Taverne, J. 1962. Some properties of the complement-fixing antigens of the agents of trachoma and inclusion blennorrhoea and the relationship of the antigens to the developmental cycle. J. Gen. Microbiol. 27: 501-508. – reference: 13. Matsumoto, A., Bessho, H., Soejima, R., and Hino, J. 1984. Biological properties of a Chlamydia strain isolated from a pet bird, budgerigar which was kept by a psittacosis patient. Kawasaki Med. J. 10: 77-90. – reference: 20. Takahashi, T., Takashima, I., and Hashimoto, N. 1988. Immunotyping of Chlamydia psittaci by indirect immunofluorescence antibody test with monoclonal antiboides. Microbiol. Immunol. 32: 251-263. – reference: 16. Peeling, R., Maclean, I. W., and Brunhan, R. C. 1984. In vitro neutralization of Chlamydia trachomatis with monoclonal antibody to an epitope on the major outer membrane protein. Infect. Immun. 46: 484-488. – reference: 6. Hackstadt, T., Todd, W. J., and Caldwell, H. D. 1985. Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of Chalmydiae? J. Bacteriol. 161: 25-31. – reference: 1. Bavoil, P., Ohlin, A., and Schachter, J. 1984. Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis. Infect. Immun. 44: 479-485. – reference: 3. Cevenini, R., Donati, M., Sambri, V., Rumpianesi, F., and La Placa, M. 1987. Reactivity of elementary and reticulate bodies of Chlamydia trachomatis LGV2 with monoclonal antibodies specific for the major outer membrane protein. FEMS Microbiol. Lett. 42: 47-51. – reference: 12. Karimi, S. T., Schloemer, R. H., and Wilde III, C. E. 1989. Accumulation of chlamydial lipopolysaccharide antigen in the plasma membranes of infected cells. Infect. Immun. 57: 1780-1785. – reference: 5. Dhir, S. P. and Boatman, E. S. 1972. Location of polysaccharide on Chlamydia psittaci by silver-methenamine staining and electron microscopy. J. Bacteriol. 111: 267-271. – reference: 7. Hatch, T. P., Miceli, M., and Sublett, J. E. 1986. Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of Chlamydia psittaci and Chlamydia trachomatis. J. Bacteriol. 165: 379-385. – reference: 22. Wang, S. -P., Kuo, C. -C., and Grayston, J. T. 1973. A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms. Infect. Immun. 7: 356-360. |
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| Snippet | Chlamydis psittaci(C. psittaci)のIzawa-1株とP-1041株をそれぞれVero E 6細胞に接種し, 単クローン性抗体を用いた間接蛍光抗体法により経時的に感染細胞におけるクラミジア抗原の産生を検討した. 属特異的なリポ多糖類抗原は全増殖過程を通じて検出された. 一方,... |
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| Title | オウム病クラミジアの増殖と各種抗原物質の出現時期に関する研究 |
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