Learning Context-Dependent DNA Mutation Patterns in Error-Prone Polymerase Chain Reaction

• Published in: Biochemistry (Easton) Vol. 62; no. 2; pp. 345 - 350
• Main Author: Nov, Yuval
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.01.2023
• Subjects: DNA - genetics; Mutation; Polymerase Chain Reaction - methods

We present a novel statistical learning method for studying context-dependent error rates in error-prone polymerase chain reaction (PCR) experiments. We demonstrate the method by applying it to error-prone PCR sequencing data and show how it may be exploited to improve the evolvability of genes in p...