Learning Context-Dependent DNA Mutation Patterns in Error-Prone Polymerase Chain Reaction

We present a novel statistical learning method for studying context-dependent error rates in error-prone polymerase chain reaction (PCR) experiments. We demonstrate the method by applying it to error-prone PCR sequencing data and show how it may be exploited to improve the evolvability of genes in p...

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Bibliographic Details
Published inBiochemistry (Easton) Vol. 62; no. 2; pp. 345 - 350
Main Author Nov, Yuval
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 17.01.2023
Subjects
DNA - genetics
Mutation
Polymerase Chain Reaction - methods
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Summary:We present a novel statistical learning method for studying context-dependent error rates in error-prone polymerase chain reaction (PCR) experiments. We demonstrate the method by applying it to error-prone PCR sequencing data and show how it may be exploited to improve the evolvability of genes in protein engineering.
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ISSN:0006-2960
1520-4995
DOI:10.1021/acs.biochem.2c00292