Dual-Functionalized Covalent Triazine Framework Nanosheets as Hierarchical Nonviral Vectors for Intracellular Gene Delivery

Gene therapy is a revolutionary biomedical technique for the treatment of many diseases, and the development of safe and efficient intracellular gene delivery vectors is crucial for successful gene therapy. Herein, we report a strategy to construct dual-functionalized covalent triazine framework-pol...

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Published inACS applied nano materials Vol. 4; no. 5; pp. 4948 - 4955
Main Authors Cao, Yaqi, Zhang, Junshi, Wang, Lan, Cen, Mingjun, Peng, Wenchao, Li, Yang, Zhang, Fengbao, Tan, Juan, Fan, Xiaobin
Format Journal Article
LanguageEnglish
Published American Chemical Society 28.05.2021
Subjects
gene delivery
covalent triazine frameworks
surface chemistry
nucleic acids
nonviral vectors
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Summary:Gene therapy is a revolutionary biomedical technique for the treatment of many diseases, and the development of safe and efficient intracellular gene delivery vectors is crucial for successful gene therapy. Herein, we report a strategy to construct dual-functionalized covalent triazine framework-poly­(ethylene glycol)-polyethylenimine (CTF-PEG-PEI) nanosheets as nonviral vectors via exfoliation and surface chemistry modification. Benefiting from the unique brushlike hierarchical structure, CTF-PEG-PEI can strongly condense nucleic acids and efficiently release the nucleic acid cargo after cellular uptake. Systematic gene delivery studies in both 293T and HeLa cells demonstrate that CTF-PEG-PEI has superior gene transfection performance and lower cytotoxicity compared with polyethylenimine (PEI), especially at low dosages.
ISSN:2574-0970
2574-0970
DOI:10.1021/acsanm.1c00477