High-moisture alfalfa silage fermentation: a comparative study on the impact of additives including formic acid, Lactobacillus plantarum , cinnamon essential oil, and wood vinegar

During the ensiling process of alfalfa, the growth and proliferation of harmful microorganisms can lead to a decline in silage quality. In this study, we report for the first time the improvement effect of cinnamon essential oil (CEO) and wood vinegar (WV) on the quality of alfalfa silage, which out...

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Published inMicrobiology spectrum p. e0000325
Main Authors Yan, Xingquan, Bao, Jian, Zhao, Muqier, Liu, Zhuotong, Wang, Mingjiu, Liu, Jingyi, Sun, Pengbo, Jia, Yushan, Ge, Gentu, Wang, Zhijun
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 23.07.2025
Subjects
Plant Microbiology
Research Article
cinnamon essential oil
bacterial community
alfalfa
wood vinegar
silage
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Summary:During the ensiling process of alfalfa, the growth and proliferation of harmful microorganisms can lead to a decline in silage quality. In this study, we report for the first time the improvement effect of cinnamon essential oil (CEO) and wood vinegar (WV) on the quality of alfalfa silage, which outperforms formic acid. This provides a reference for the development of novel silage additives. The results indicate that the addition of Lactobacillus plantarum , CEO, and WV can enhance the abundance of beneficial microorganisms, including Lactobacillus , Pediococcus , Lactococcus , and Weissella , while inhibiting the growth of harmful microorganisms such as Pantoea , thereby effectively improving the quality and safety of alfalfa silage.
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ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.00003-25