Accelerating Diverse Cell-Based Therapies Through Scalable Design

Augmenting cells with novel, genetically encoded functions will support therapies that expand beyond natural capacity for immune surveillance and tissue regeneration. However, engineering cells at scale with transgenic cargoes remains a challenge in realizing the potential of cell-based therapies. I...

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Published inAnnual review of chemical and biomolecular engineering Vol. 15; no. 1; pp. 267 - 292
Main Authors Peterman, Emma L, Ploessl, Deon S, Galloway, Kate E
Format Journal Article
LanguageEnglish
Published United States Annual Reviews 24.07.2024
Subjects
biomanufacturing
cell-based therapies
cellular engineering
genome editing
stem cells
synthetic biology
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Summary:Augmenting cells with novel, genetically encoded functions will support therapies that expand beyond natural capacity for immune surveillance and tissue regeneration. However, engineering cells at scale with transgenic cargoes remains a challenge in realizing the potential of cell-based therapies. In this review, we introduce a range of applications for engineering primary cells and stem cells for cell-based therapies. We highlight tools and advances that have launched mammalian cell engineering from bioproduction to precision editing of therapeutically relevant cells. Additionally, we examine how transgenesis methods and genetic cargo designs can be tailored for performance. Altogether, we offer a vision for accelerating the translation of innovative cell-based therapies by harnessing diverse cell types, integrating the expanding array of synthetic biology tools, and building cellular tools through advanced genome writing techniques.
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ISSN:1947-5438
1947-5446
1947-5446
DOI:10.1146/annurev-chembioeng-100722-121610