カイコ核多角体病ウイルスの還元剤による不活化

Reducing agents such as glutathione and ascorbic acid inactivated a nucleopolyhedrovirus (NPV) of the silkworm in vitro. Activity of Bombyx mori NPV virions was lost after incubation with 5 mM reduced glutathione or 5 mM ascorbic acid at 25℃ for 1 day. A half time of inactivation at 0.05% (2.8 mM) o...

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Published inNihon sanshigaku zasshi Vol. 72; no. 2; pp. 49 - 54
Main Authors 中尾, 亮介, 永田, 昌男, 浜田, 幸治, 青木, 不学
Format Journal Article
LanguageJapanese
Published 社団法人 日本蚕糸学会 2003
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ISSN0037-2455
1884-796X
DOI10.11416/kontyushigen.72.49

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Summary:Reducing agents such as glutathione and ascorbic acid inactivated a nucleopolyhedrovirus (NPV) of the silkworm in vitro. Activity of Bombyx mori NPV virions was lost after incubation with 5 mM reduced glutathione or 5 mM ascorbic acid at 25℃ for 1 day. A half time of inactivation at 0.05% (2.8 mM) of ascorbic acids was estimated to be 1.5 minutes. NPV in the hemolymph from NPV-infected larvae was inactivated after treatment with 1% ascorbic acid at 25℃ for 1 day. From these results, applications of ascorbic acid to disinfection against silkworm viruses were discussed.
Bibliography:680281
ZZ00018606
ISSN:0037-2455
1884-796X
DOI:10.11416/kontyushigen.72.49