Non-invasive Point-of-Care Device To Diagnose Acute Mesenteric Ischemia

• Published in: ACS sensors Vol. 3; no. 11; pp. 2296 - 2302
• Main Authors: Abdelrasoul, Gaser N, MacKay, Scott, Salim, Saad Y, Ismond, Kathleen P, Tamura, Marcus, Khalifa, Charfeddine, Mannan, Emma, Lin, Donghai, Mandal, Tanushree, Montgomery, Ruth R, Wishart, David S, Chen, Jie, Khadaroo, Rachel G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26.11.2018
• Subjects: point-of-care device; non-invasive urine test; bedside monitoring; impedance-based biosensor; acute mesenteric ischemia
• ISSN: 2379-3694; 2379-3694
• DOI: 10.1021/acssensors.8b00558

Inadequate blood supply to the intestine can lead to acute mesenteric ischemia (AMI), with a mortality rate ranging from 60% to 90%. This high mortality rate is partially due to late detection and the lack of efficient early diagnostic tests. There is an urgent need for a point-of-care tool for immediate bedside diagnosis. Here we present for the first time a rapid and non-invasive electrochemical biosensor device based on non-faradic impedance spectroscopy to detect intestinal fatty-acid binding protein (I-FABP) as an indication of AMI. The electrochemical biosensors consist of gold interdigitated electrodes that were fabricated using photolithographic techniques on top of silicon dioxide substrates. The electrode surfaces were functionalized with an I-FABP capture antibody (CAnB) to entice the target protein, while gold nanoparticles (GNPs) functionalized with detection antibodies (DAnB-GNPs) were utilized as a novel mechanism to enhance the detection signal. Quantification of the I-FABP concentration in the medium depended on its attachment to CAnB and DAnB-GNPs in a sandwich manner, where the latter boosts the impedance signal through its binding to the I-FABP. This non-invasive non-faradic electric biosensor device demonstrates the potential for bench-to-bedside translation with the goal of decreasing morbidity and mortality from AMI.