Exploiting Photoelectric Activities and Piezoelectric Properties of NaNbO3 Semiconductors for Point-of-Care Immunoassay

• Published in: Analytical chemistry (Washington) Vol. 94; no. 7; pp. 3418 - 3426
• Main Authors: Yu, Zhichao, Gong, Hexiang, Xu, Jianhui, Li, Yuxuan, Zeng, Yongyi, Liu, Xiaolong, Tang, Dianping
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 22.02.2022
• Subjects: Antigens; Ascorbic acid; Biosensors; Body fluids; Chemistry; Commercialization; Downsizing; Electron transfer; Electronics industry; Enzyme-linked immunosorbent assay; Heterojunctions; Immune system; Immunoassay; Photoelectricity; Piezoelectricity; Prostate; Prostate-specific antigen; Semiconductors; Substrates; Test stands
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.2c00066

Point-of-care testing (POCT) technology has made major breakthroughs in community medicine and physician office situations, in tandem with the more ubiquitous and intensive usage of highly integrated quick detection equipment for illness diagnosis, personal care, and mobile healthcare. Although the photoelectrochemical (PEC)-based POCT platform offers the benefits of cheap cost and good user engagement, its commercialization is still limited by the photodetection components’ downsizing and mobility, among other factors. In this work, a novel highly integrated PEC biosensor aided by piezophototronics to enhance the efficiency of PEC testing was reported for flexible detection of cancer-associated antigens in biological fluids (prostate-specific antigen, PSA, used as an example). Multiple signal enhancement strategies, including a magnetic bead-linked enzyme-linked immune system catalyzing the production of ascorbic acid from the substrate and a piezoelectric-assisted enhancement strategy, were used for sensitive detection of the analyte to be tested in human body fluids. Unlike the electron transfer mechanism in heterojunctions, piezoelectric semiconductors promote the transfer of electrons and holes by generating piezoelectric potentials in the ultrasonic field, thus contributing to the performance of the PEC testbed. Under optimized conditions, the test platform achieves good correspondence for PSA at 0.02–40 ng mL–1. Impressively, the test devices are comparable to or even superior to gold standard ELISA kits in terms of cost approval and batch testing. This research demonstrates the potential of piezoelectric semiconductors for POC applications in revolutionary PECs and offers innovative thoughts for the development of new PEC bioanalytical components.