NMR Immunosensor Based on a Targeted Gadolinium Nanoprobe for Detecting Salmonella in Milk

• Published in: Analytical chemistry (Washington) Vol. 96; no. 28; pp. 11334 - 11342
• Main Authors: Guo, Mengdi, Yi, Zhibin, Li, Huo, Liu, Yang, Ding, Liping, Babailov, Sergey P., Xiong, Chunhong, Huang, Ganhui, Zhang, Jinsheng
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.07.2024
• Subjects: Biosensors; Chelation; Food; Food quality; Foodborne pathogens; Gadolinium; Immunosensors; Magnetic properties; NMR; Nuclear magnetic resonance; Public health; Quality management; Salmonella; Streptavidin
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.4c01265

Detecting harmful pathogens in food is not only a crucial aspect of food quality management but also an effective way to ensure public health. In this paper, a complete nuclear magnetic resonance biosensor based on a novel gadolinium (Gd)-targeting molecular probe was developed for the detection of Salmonella in milk. First, streptavidin was conjugated to the activated macromolecular polyaspartic acid (PASP) via an amide reaction to generate SA–PASP. Subsequently, the strong chelating and adsorption properties of PASP toward the lanthanide metal gadolinium ions were exploited to generate the magnetic complex (SA–PASP–Gd). Finally, the magnetic complex was linked to biotinylated antibodies to obtain the bioprobe and achieve the capture of Salmonella. Under optimal experimental conditions, the sensor we have constructed can achieve a rapid detection of Salmonella within 1.5 h, with a detection limit of 7.1 × 103 cfu mL–1.