Engineering Entomopathogenic Fungi Using Thermal-Responsive Polymer to Boost Their Resilience against Abiotic Stresses

• Published in: Journal of agricultural and food chemistry Vol. 72; no. 37; pp. 20308 - 20320
• Main Authors: Yang, Guang, Bi, Feihu, Yu, Deshui, Wang, Yulong, Ren, Hui, Wei, Hanchen, Wang, Zhangxun, Huang, Bo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 18.09.2024
• Subjects: Agricultural and Environmental Chemistry; Animals; Hot Temperature; Metarhizium - chemistry; Metarhizium - genetics; Metarhizium - radiation effects; Pest Control, Biological; Polymers - chemistry; Polymers - pharmacology; Spores, Fungal - drug effects; Spores, Fungal - radiation effects; Stress, Physiological; Ultraviolet Rays; abiotic stresses; Metarhizium robertsii; entomopathogenic fungi; thermal-responsive polymers
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.4c04400

Entomopathogenic fungi offer an ecologically sustainable and highly effective alternative to chemical pesticides for managing plant pests. However, the efficacy of mycoinsecticides in pest control suffers from environmental abiotic stresses, such as solar UV radiation and temperature fluctuations, which seriously hinder their practical application in the field. Herein, we discovered that the synthetic amphiphilic thermal-responsive polymers are able to significantly enhance the resistance of Metarhizium robertsii conidia against thermal and UV irradiation stresses. The thermosensitive polymers with extremely low cytotoxicity and good biocompatibility can be engineered onto the M. robertsii conidia surface by anchoring hydrophobic alkyl chains. Further investigations revealed that polymer supplementation remarkably augmented the capacity for penetration and the virulence of M. robertsii under heat and UV stresses. Notably, broad-spectrum entomopathogenic fungi can be protected by the polymers. The molecular mechanism was elucidated through exploring RNA sequencing and in vivo/vitro enzyme activity assays. This work provides a novel avenue for fortifying the resilience of entomopathogenic fungi, potentially advancing their practical application as biopesticides.