Epitope-Shared Hapten Enhancing Antibody Polyreactivity for Simultaneous Immunochromatography of Oxyphenisatin Adulterants

• Published in: Journal of agricultural and food chemistry Vol. 72; no. 24; pp. 13978 - 13988
• Main Authors: Liu, Zhiwei, Peng, Longpeng, Li, Zhaodong, Yuanzhu, Zeyang, Abbas Raza, Sayed Haidar, Zhang, Shiwei, Lei, Yi, Pan, Liangwen, Yu, Xiaoqin, Lei, Hongtao
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.06.2024
• Subjects: Food Safety and Toxicology; hapten prediction; antibody recognition mechanism; immunochromatography; oxyphenisatin adulterants; weight-loss foods
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.4c02855

Given the significant threat posed by oxyphenisatin adulterants (OPHs) in weight-loss foods, simultaneous analysis of the OPHs is necessary. Herein, four novel haptens based on the general epitope shared among the OPHs were raised by computer-aided chemical modeling prediction, with the expectation of eliciting antibody responses targeting three of the OPHs. One obtained monoclonal antibody (mAb) showed maximal half-inhibitory concentration (IC50) of 0.40–12.11 ng/mL for OPHs. The key interaction forces responsible for the corecognition of the OPHs were revealed by the intrinsic molecular mechanism. The developed immunochromatography (ICA) indicated a detection capability for screening (CCβ) for OPHs estimated to be 5–600 ng/g in jelly, candy tablets, and oral liquid. Furthermore, the analysis of 15 real samples by our method showed a good correlation with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our research not only presented a rapid approach for identifying OPHs adulteration but also proposed an effective hapten prediction strategy to enhance antibody polyreactivity.