Perichondrium-Wrapped Collagenous Matrices to Induce Chondroneogenesis: An In Vitro Study

• Published in: Archives of facial plastic surgery Vol. 3; no. 2; pp. 122 - 126
• Main Authors: van Lange, Jeroen W. L, de Roo, Kirsten, Middelkoop, Esther, van den Bos, Theo, Everts, Vincent, Nolst, Trenité Gilbert J
• Format: Journal Article
• Language: English
• Published: United States American Medical Association 01.04.2001
• Subjects: Animals; Bioartificial Organs; Biocompatible Materials; Cartilage - cytology; Cartilage - metabolism; Cells, Cultured; Chondrogenesis; Collagen; Connective Tissue Cells - cytology; Connective Tissue Cells - metabolism; Female; Immunohistochemistry; Proline - metabolism; Rabbits
• ISSN: 1521-2491; 2168-6076; 1538-3660; 2168-6092
• DOI: 10.1001/archfaci.3.2.122

Objective  To develop a model for cartilage regeneration in vitro, to be used for cartilage reconstruction in vivo.Methods  Collagenous matrices were wrapped in a perichondrium layer. The matrices served as carriers to allow migration of cells from the perichondrium into the matrix. Culture conditions stimulated cell growth and proliferation.Results  After 4 weeks of culturing, microscopic examination showed an increase of cell layers around the matrices but also of cells migrated into porous matrices. Immunohistochemical staining of these cultured cells expressed type II collagen intracellularly.Conclusions  This model seems appropriate to culture mucoperichondrial explants in combination with collagenous matrices. Cells migrate into the pores of the matrix, survive, and synthesize matrix components. Actual formation of cartilage has not been shown to occur. Adding growth factors to this model may influence induction of this activity.