AbSE Workflow: Rapid Identification of the Coding Sequence and Linear Epitope of the Monoclonal Antibody at the Single-cell Level

• Published in: ACS synthetic biology Vol. 11; no. 5; pp. 1856 - 1864
• Main Authors: Ma, Lerong, Ouyang, HongSheng, Su, Ang, Zhang, Yuanzhu, Pang, Daxin, Zhang, Tao, Sun, Ruize, Wang, Wentao, Xie, Zicong, Lv, Dongmei
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.05.2022
• Subjects: linear epitope; de novo assembly; single-cell RNA-seq (scRNAseq); antibody sequence; PhIP-Seq
• ISSN: 2161-5063; 2161-5063
• DOI: 10.1021/acssynbio.2c00018

Monoclonal antibody (mAb) has been widely used in immunity research and disease diagnosis and therapy. Antibody sequence and epitope are the prerequisites and basis of mAb applications, which determine the properties of antibodies and make the preparation of antibody-based molecules controllable and reliable. Here, we present the antibody sequence and epitope identification (AbSE) workflow, a time-saving and cost-effective route for rapid determination of antibody sequence and linear epitope of mAb even at the single-cell level. The feasibility and accuracy of the AbSE workflow were demonstrated through the identification and validation of the coding sequence and epitope of antihuman serum albumin (antiHSA) mAb. It can be inferred that the AbSE workflow is a powerful and universal approach for paired antibody–epitope sequence identification. It may characterize antibodies not only on a single hybridoma cell but also on any other antibody-secreting cells.